Detailed information of TA system TA023231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 481703..482358
• Replicon: chromosome
• Accession: NC_011527
• Organism: Coxiella burnetii CbuG Q212

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CBUG_RS02585
• Protein ID: WP_011996617.1
• Coordinates: 481703..482041 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CBUG_RS02590
• Protein ID: WP_010958263.1
• Coordinates: 482047..482358 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBUG_RS02570	477427..478182	+	756	WP_012569800.1	pyridoxine 5'-phosphate synthase	-
CBUG_RS02575	478358..480023	-	1666	Protein_509	CBU_1493 family Dot/Icm T4SS effector	-
CBUG_RS02580	480199..481344	-	1146	WP_012569768.1	ISAs1-like element ISCbu1 family transposase	-
CBUG_RS02585	481703..482041	+	339	WP_011996617.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CBUG_RS02590	482047..482358	+	312	WP_010958263.1	HigA family addiction module antidote protein	Antitoxin
CBUG_RS02595	482428..483159	-	732	WP_012569802.1	UDP-2,3-diacylglucosamine diphosphatase	-
CBUG_RS02600	483316..484713	+	1398	WP_012569803.1	glutamate--tRNA ligase	-
CBUG_RS02605	484704..486086	+	1383	WP_010958260.1	cysteine--tRNA ligase	-
CBUG_RS02615	486406..487083	+	678	WP_012569804.1	cyclin protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	480199..481344	1145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (20-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (26-72)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13428.63 Da
• Isoelectric Point: 10.2262

>T23231 WP_011996617.1 NC_011527:481703-482041 [Coxiella burnetii CbuG_Q212]
MPLTLYIMLDVTRKTVILEVMIKSFKDKYTKYLYEGVSVSKWQAIRKQAERRLQILDSVTSLDDLRSLPSNRFESLRGNR
KGQFSIRINKQWRICFKWINNEPTEVEIVDYH

Nucleotide

• Length: 339 bp

>T23231 NC_011527:481703-482041 [Coxiella burnetii CbuG_Q212]
TTGCCTCTTACGCTGTATATTATGCTTGATGTAACGCGTAAAACTGTTATCCTTGAAGTGATGATAAAAAGTTTCAAGGA
CAAATACACTAAATATTTATATGAAGGTGTATCCGTTTCAAAATGGCAAGCCATACGAAAGCAGGCGGAAAGACGGCTTC
AGATTTTGGATTCAGTGACATCGTTAGATGATTTGAGAAGTTTGCCAAGCAATCGTTTTGAATCGTTGCGGGGGAATAGA
AAAGGACAATTTAGTATAAGGATTAATAAGCAATGGCGCATTTGTTTTAAGTGGATTAACAATGAGCCAACTGAAGTTGA
AATTGTGGATTATCATTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11458.39 Da
• Isoelectric Point: 10.1699

>AT23231 WP_010958263.1 NC_011527:482047-482358 [Coxiella burnetii CbuG_Q212]
MKMAANRMRPIHPGEILAEELGFLDKMSANQLAKHLAIPTNRVTAILNGARSITADTALRLAKFFGTTPEFWLNLQDAYD
IKMALKKSGKKIEKEVTPYDQAA

Nucleotide

• Length: 312 bp

>AT23231 NC_011527:482047-482358 [Coxiella burnetii CbuG_Q212]
ATGAAAATGGCAGCTAATAGAATGCGACCCATACATCCAGGTGAGATCCTTGCTGAAGAGTTGGGGTTTTTAGATAAGAT
GTCAGCTAATCAGTTAGCTAAACATTTAGCAATTCCGACCAACCGAGTGACCGCCATTCTCAATGGCGCGCGATCCATTA
CGGCGGATACCGCGCTACGATTGGCTAAGTTTTTTGGGACGACGCCGGAATTTTGGCTAAATCTCCAAGACGCCTATGAC
ATAAAAATGGCATTGAAAAAGTCCGGCAAAAAAATCGAAAAAGAAGTAACCCCTTACGACCAAGCCGCCTAG