Detailed information of TA system TA232293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1631..2154
• Replicon: plasmid pSER10C-1
• Accession: NZ_CP091007
• Organism: Staphylococcus epidermidis strain R10C

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A8B5RPI4
• Locus tag: KB224_RS11945
• Protein ID: WP_029376511.1
• Coordinates: 1631..1897 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: C5QR06
• Locus tag: KB224_RS11950
• Protein ID: WP_002441972.1
• Coordinates: 1897..2154 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB224_RS11930 (KB224_11930)	1..357	+	357	WP_002441965.1	hypothetical protein	-
KB224_RS11935 (KB224_11935)	858..1385	-	528	WP_080280668.1	cellulase family glycosylhydrolase	-
KB224_RS11940 (KB224_11940)	1444..1614	-	171	WP_171049635.1	hypothetical protein	-
KB224_RS11945 (KB224_11945)	1631..1897	-	267	WP_029376511.1	Txe/YoeB family addiction module toxin	Toxin
KB224_RS11950 (KB224_11950)	1897..2154	-	258	WP_002441972.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KB224_RS11955 (KB224_11955)	2317..2556	+	240	WP_227946811.1	YolD-like family protein	-
KB224_RS11960 (KB224_11960)	2570..2716	+	147	WP_227946810.1	YolD-like family protein	-
KB224_RS11965 (KB224_11965)	2749..2928	-	180	WP_002456267.1	CsbD family protein	-
KB224_RS11970 (KB224_11970)	3325..3471	+	147	WP_002441983.1	hypothetical protein	-
KB224_RS11975 (KB224_11975)	4318..4698	+	381	WP_235063564.1	hypothetical protein	-
KB224_RS11980 (KB224_11980)	4700..5083	+	384	WP_029376512.1	hypothetical protein	-
KB224_RS11985 (KB224_11985)	5095..5916	+	822	WP_049374124.1	relaxase MobL	-
KB224_RS11990 (KB224_11990)	6827..6982	+	156	WP_002452621.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..43056	43056

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10721.38 Da
• Isoelectric Point: 10.3481

>T232293 WP_029376511.1 NZ_CP091007:c1897-1631 [Staphylococcus epidermidis]
MAKYKINIKNSAKTDLKKIKRSHLQEQFLKIVEILKYNPYQQSQSFEKLHPKHLGRYSRRINHQHRVVYTIDEENKEVLI
LATWSHYE

Nucleotide

• Length: 267 bp

>T232293 NZ_CP127275:c3049695-3049279 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 10041.10 Da
• Isoelectric Point: 4.5610

>AT232293 WP_002441972.1 NZ_CP091007:c2154-1897 [Staphylococcus epidermidis]
MKSKTPTEARKDFYQLLKRVNNDHEPVYIEGKYDESNAVIIGMEDWRSINETIYLEATGTMDKVREREHDNSGYTNIDDI
DWARL

Nucleotide

• Length: 258 bp

>AT232293 NZ_CP127275:c3049958-3049692 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: A0A8B5RPI4

Antitoxin

• AlphaFold DB: A0A8B5RUG0