Detailed information of TA system TA232220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5048111..5048700
• Replicon: chromosome
• Accession: NZ_CP090954
• Organism: Xanthomonas arboricola pv. pruni strain Xcp1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2S7AZR2
• Locus tag: L1B16_RS21690
• Protein ID: WP_039429225.1
• Coordinates: 5048419..5048700 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L1B16_RS21685
• Protein ID: WP_006449989.1
• Coordinates: 5048111..5048401 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1B16_RS21665 (L1B16_10330)	5044253..5045089	+	837	WP_081327584.1	restriction endonuclease	-
L1B16_RS21670 (L1B16_10335)	5045164..5047062	+	1899	WP_126750750.1	hypothetical protein	-
L1B16_RS21675 (L1B16_10340)	5047236..5047568	+	333	WP_039513672.1	lipoprotein	-
L1B16_RS21680 (L1B16_10345)	5047718..5047920	+	203	Protein_4241	hypothetical protein	-
L1B16_RS21685 (L1B16_10350)	5048111..5048401	-	291	WP_006449989.1	HigA family addiction module antitoxin	Antitoxin
L1B16_RS21690 (L1B16_10355)	5048419..5048700	-	282	WP_039429225.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L1B16_RS21695 (L1B16_10360)	5048840..5050966	-	2127	WP_043087609.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11046.72 Da
• Isoelectric Point: 9.7972

>T232220 WP_039429225.1 NZ_CP090954:c5048700-5048419 [Xanthomonas arboricola pv. pruni]
MIRSFVDKEAEKIWLGERSRRLPADIQSVARRKLRMLNAAAHLDDLRIPPANRLEALKGERRGQYSIRINDQWRICFRWM
EGDVAEVEIVDYH

Nucleotide

• Length: 282 bp

>T232220 NZ_CP127274:c2209766-2209347 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10540.20 Da
• Isoelectric Point: 6.4664

>AT232220 WP_006449989.1 NZ_CP090954:c5048401-5048111 [Xanthomonas arboricola pv. pruni]
MTVLPNIHPGEILLEEFLEPMGISQNALARATDVPPRRINEIVLGKRGITADTAVRLAAALGTTERFWLGLQADYELEQA
HRALGDLPSRIKRLAA

Nucleotide

• Length: 291 bp

>AT232220 NZ_CP127274:c2210035-2209775 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Toxin

• AlphaFold DB: A0A2S7AZR2