Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4623362..4623859 Replicon chromosome
Accession NC_011415
Organism Escherichia coli SE11

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECSE_RS23030 Protein ID WP_001173343.1
Coordinates 4623686..4623859 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECSE_RS23025 Protein ID WP_000398619.1
Coordinates 4623362..4623658 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSE_RS23005 4620093..4620812 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECSE_RS23010 4620809..4622440 - 1632 WP_001216460.1 sensor histidine kinase -
ECSE_RS23015 4622621..4622851 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
ECSE_RS23020 4622863..4623135 + 273 WP_000405647.1 N-acetyltransferase -
ECSE_RS23025 4623362..4623658 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECSE_RS23030 4623686..4623859 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECSE_RS23035 4623978..4625495 - 1518 WP_001295074.1 lysine--tRNA ligase -
ECSE_RS23040 4625732..4627189 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4623362..4632712 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T23222 WP_001173343.1 NC_011415:4623686-4623859 [Escherichia coli SE11]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T23222 NC_011415:4623686-4623859 [Escherichia coli SE11]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT23222 WP_000398619.1 NC_011415:4623362-4623658 [Escherichia coli SE11]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT23222 NC_011415:4623362-4623658 [Escherichia coli SE11]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References