Detailed information of TA system TA232215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 29365..29888
• Replicon: plasmid unnamed1
• Accession: NZ_CP090942
• Organism: Staphylococcus epidermidis strain HAF242

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LZT96_RS12165
• Protein ID: WP_002456264.1
• Coordinates: 29365..29631 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: C5QR06
• Locus tag: LZT96_RS12170
• Protein ID: WP_002441972.1
• Coordinates: 29631..29888 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZT96_RS12140 (LZT96_12140)	25810..25887	+	78	WP_225088349.1	putative holin-like toxin	-
LZT96_RS12145 (LZT96_12145)	26874..27422	-	549	WP_002498726.1	recombinase family protein	-
LZT96_RS12150 (LZT96_12150)	27736..28092	+	357	WP_002441965.1	hypothetical protein	-
LZT96_RS12155 (LZT96_12155)	28593..28817	-	225	WP_225981062.1	hypothetical protein	-
LZT96_RS12160 (LZT96_12160)	29178..29348	-	171	WP_002456263.1	hypothetical protein	-
LZT96_RS12165 (LZT96_12165)	29365..29631	-	267	WP_002456264.1	Txe/YoeB family addiction module toxin	Toxin
LZT96_RS12170 (LZT96_12170)	29631..29888	-	258	WP_002441972.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LZT96_RS12175 (LZT96_12175)	30051..30449	+	399	WP_002498728.1	YolD-like family protein	-
LZT96_RS12180 (LZT96_12180)	30482..30661	-	180	WP_002456267.1	CsbD family protein	-
LZT96_RS12185 (LZT96_12185)	31058..31204	+	147	WP_002441983.1	hypothetical protein	-
LZT96_RS12190 (LZT96_12190)	32074..32430	+	357	WP_225981063.1	hypothetical protein	-
LZT96_RS12195 (LZT96_12195)	32432..32815	+	384	WP_002456270.1	hypothetical protein	-
LZT96_RS12200 (LZT96_12200)	32827..33648	+	822	WP_002456271.1	relaxase MobL	-
LZT96_RS12205 (LZT96_12205)	34559..34714	+	156	WP_002452621.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..64388	64388

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10722.36 Da
• Isoelectric Point: 10.2282

>T232215 WP_002456264.1 NZ_CP090942:c29631-29365 [Staphylococcus epidermidis]
MAKYKINIKNSAKTDLKKIKRSHLQEQFLKIVEILKYDPYQQSQSFEKLHPKHLGRYSRRINHQHRVVYTIDEENKEVLI
LATWSHYE

Nucleotide

• Length: 267 bp

>T232215 NZ_CP127274:c1938186-1937797 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 10041.10 Da
• Isoelectric Point: 4.5610

>AT232215 WP_002441972.1 NZ_CP090942:c29888-29631 [Staphylococcus epidermidis]
MKSKTPTEARKDFYQLLKRVNNDHEPVYIEGKYDESNAVIIGMEDWRSINETIYLEATGTMDKVREREHDNSGYTNIDDI
DWARL

Nucleotide

• Length: 258 bp

>AT232215 NZ_CP127274:c1938410-1938183 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A8B5RUG0