Detailed information of TA system TA023221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4339825..4340427
• Replicon: chromosome
• Accession: NC_011415
• Organism: Escherichia coli SE11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: ECSE_RS21680
• Protein ID: WP_000897305.1
• Coordinates: 4339825..4340136 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECSE_RS21685
• Protein ID: WP_000356397.1
• Coordinates: 4340137..4340427 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSE_RS21650	4334855..4335640	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
ECSE_RS21655	4335739..4336338	+	600	WP_001295269.1	glucose-1-phosphatase	-
ECSE_RS21660	4336332..4337204	+	873	WP_000920762.1	virulence factor BrkB family protein	-
ECSE_RS21665	4337201..4337638	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ECSE_RS21670	4337683..4338624	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ECSE_RS21675	4338688..4339596	-	909	WP_001162704.1	alpha/beta hydrolase	-
ECSE_RS21680	4339825..4340136	+	312	WP_000897305.1	hypothetical protein	Toxin
ECSE_RS21685	4340137..4340427	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ECSE_RS21695	4341013..4341231	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
ECSE_RS21700	4341450..4341692	+	243	WP_001086388.1	hypothetical protein	-
ECSE_RS21710	4342022..4342951	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
ECSE_RS21715	4342948..4343583	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
ECSE_RS21720	4343580..4344482	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T23221 WP_000897305.1 NC_011415:4339825-4340136 [Escherichia coli SE11]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T23221 NC_011415:4339825-4340136 [Escherichia coli SE11]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT23221 WP_000356397.1 NC_011415:4340137-4340427 [Escherichia coli SE11]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT23221 NC_011415:4340137-4340427 [Escherichia coli SE11]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7