Detailed information of TA system TA023219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3544806..3545605
• Replicon: chromosome
• Accession: NC_011415
• Organism: Escherichia coli SE11

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: ECSE_RS17750
• Protein ID: WP_000347266.1
• Coordinates: 3545141..3545605 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: ECSE_RS17745
• Protein ID: WP_001307405.1
• Coordinates: 3544806..3545141 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSE_RS17725	3540591..3541361	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
ECSE_RS17730	3541377..3542711	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
ECSE_RS17740	3543086..3544657	+	1572	WP_001273741.1	galactarate dehydratase	-
ECSE_RS17745	3544806..3545141	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
ECSE_RS17750	3545141..3545605	+	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
ECSE_RS17755	3545660..3546469	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
ECSE_RS17760	3546718..3547998	+	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
ECSE_RS17765	3548021..3548494	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
ECSE_RS17770	3548505..3549284	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
ECSE_RS17775	3549274..3550152	+	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
ECSE_RS17780	3550170..3550604	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3534150..3545605	11455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T23219 WP_000347266.1 NC_011415:3545141-3545605 [Escherichia coli SE11]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T23219 NC_011415:3545141-3545605 [Escherichia coli SE11]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT23219 WP_001307405.1 NC_011415:3544806-3545141 [Escherichia coli SE11]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT23219 NC_011415:3544806-3545141 [Escherichia coli SE11]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A836NGD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7