Detailed information of TA system TA232135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 38716..39239
• Replicon: plasmid pSE45A5-1
• Accession: NZ_CP090916
• Organism: Staphylococcus epidermidis strain 45A5

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: C5QR05
• Locus tag: KB229_RS12260
• Protein ID: WP_002441971.1
• Coordinates: 38973..39239 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: KB229_RS12255
• Protein ID: WP_162159478.1
• Coordinates: 38716..38973 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB229_RS12215 (KB229_12215)	34255..34638	-	384	WP_059224731.1	hypothetical protein	-
KB229_RS12220 (KB229_12220)	34640..34918	-	279	WP_017464867.1	hypothetical protein	-
KB229_RS12225 (KB229_12225)	35766..36089	-	324	WP_234966607.1	hypothetical protein	-
KB229_RS12230 (KB229_12230)	36345..36620	-	276	WP_234966609.1	helix-turn-helix transcriptional regulator	-
KB229_RS12235 (KB229_12235)	36604..36981	-	378	WP_234966611.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KB229_RS12240 (KB229_12240)	37189..37431	-	243	WP_234966613.1	hypothetical protein	-
KB229_RS12245 (KB229_12245)	37941..38120	+	180	WP_002457008.1	CsbD family protein	-
KB229_RS12250 (KB229_12250)	38153..38551	-	399	WP_162159479.1	YolD-like family protein	-
KB229_RS12255 (KB229_12255)	38716..38973	+	258	WP_162159478.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KB229_RS12260 (KB229_12260)	38973..39239	+	267	WP_002441971.1	Txe/YoeB family addiction module toxin	Toxin
KB229_RS12265 (KB229_12265)	39256..39426	+	171	WP_016898857.1	hypothetical protein	-
KB229_RS12270 (KB229_12270)	39733..40044	+	312	WP_162159477.1	hypothetical protein	-
KB229_RS12275 (KB229_12275)	40342..40890	+	549	WP_234966615.1	recombinase family protein	-
KB229_RS12280 (KB229_12280)	41081..41680	+	600	WP_234966617.1	CPBP family intramembrane metalloprotease	-
KB229_RS12285 (KB229_12285)	41843..41929	-	87	WP_234966660.1	hypothetical protein	-
KB229_RS12290 (KB229_12290)	42130..43398	-	1269	WP_234966619.1	penicillin-binding protein PBP4	-
KB229_RS12295 (KB229_12295)	43678..44079	-	402	WP_234966621.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..53974	53974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10692.34 Da
• Isoelectric Point: 10.2282

>T232135 WP_002441971.1 NZ_CP090916:38973-39239 [Staphylococcus epidermidis]
MAKYKINIKNSAKTDLKKIKRSHLQEQFLKIVEILKYDPYQQSQSFEKLHPKHLGRYSRRINHQHRVVYTIDEENKEVLI
LAAWSHYE

Nucleotide

• Length: 267 bp

>T232135 NZ_CP127273:677230-677637 [Mycobacterium tuberculosis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9983.06 Da
• Isoelectric Point: 4.6899

>AT232135 WP_162159478.1 NZ_CP090916:38716-38973 [Staphylococcus epidermidis]
MKSKTPTEARKDFYQLLKRVNNDHEPVYIEGKYDESNAVIIGMEDWRSINETIYLEATGTMDKVREREHDNSGYTNIGDI
DWARL

Nucleotide

• Length: 258 bp

>AT232135 NZ_CP127273:677018-677233 [Mycobacterium tuberculosis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA