Detailed information of TA system TA232121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3931229..3931956
• Replicon: chromosome
• Accession: NZ_CP090909
• Organism: Enterobacter hormaechei strain M1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B3V067
• Locus tag: L1E13_RS18610
• Protein ID: WP_022650547.1
• Coordinates: 3931645..3931956 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L1E13_RS18605
• Protein ID: WP_032652900.1
• Coordinates: 3931229..3931648 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1E13_RS18590 (L1E13_18590)	3928301..3929422	-	1122	WP_234978885.1	YbdK family carboxylate-amine ligase	-
L1E13_RS18595 (L1E13_18595)	3929528..3930418	+	891	WP_234978886.1	oxidoreductase	-
L1E13_RS18600 (L1E13_18600)	3930471..3930905	-	435	WP_023315573.1	hypothetical protein	-
L1E13_RS18605 (L1E13_18605)	3931229..3931648	-	420	WP_032652900.1	helix-turn-helix domain-containing protein	Antitoxin
L1E13_RS18610 (L1E13_18610)	3931645..3931956	-	312	WP_022650547.1	type II toxin-antitoxin system HigB family toxin	Toxin
L1E13_RS18615 (L1E13_18615)	3932412..3933068	+	657	WP_022650548.1	hypothetical protein	-
L1E13_RS18620 (L1E13_18620)	3933182..3933637	-	456	WP_165729691.1	hypothetical protein	-
L1E13_RS18625 (L1E13_18625)	3933928..3934809	-	882	WP_058659370.1	LysR family transcriptional regulator	-
L1E13_RS18630 (L1E13_18630)	3934915..3935694	+	780	WP_003858848.1	acetolactate decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12554.34 Da
• Isoelectric Point: 9.9435

>T232121 WP_022650547.1 NZ_CP090909:c3931956-3931645 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Nucleotide

• Length: 312 bp

>T232121 NZ_CP127255:c4672801-4672466 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15437.48 Da
• Isoelectric Point: 4.6051

>AT232121 WP_032652900.1 NZ_CP090909:c3931648-3931229 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESSEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Nucleotide

• Length: 420 bp

>AT232121 NZ_CP127255:c4673049-4672801 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A8B3V067