Detailed information of TA system TA232106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4839398..4840128
• Replicon: chromosome
• Accession: NZ_CP090908
• Organism: Serratia marcescens strain Sys06

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0P0QJB7
• Locus tag: L1F17_RS23330
• Protein ID: WP_060560355.1
• Coordinates: 4839398..4839709 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L1F17_RS23335
• Protein ID: WP_033632013.1
• Coordinates: 4839706..4840128 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1F17_RS23300 (L1F17_23300)	4834725..4835450	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
L1F17_RS23305 (L1F17_23305)	4835503..4836936	+	1434	WP_021505109.1	RNA polymerase factor sigma-54	-
L1F17_RS23310 (L1F17_23310)	4836960..4837247	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
L1F17_RS23315 (L1F17_23315)	4837403..4837864	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
L1F17_RS23320 (L1F17_23320)	4837992..4838846	+	855	WP_015379177.1	RNase adapter RapZ	-
L1F17_RS23325 (L1F17_23325)	4838843..4839115	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
L1F17_RS23330 (L1F17_23330)	4839398..4839709	+	312	WP_060560355.1	type II toxin-antitoxin system HigB family toxin	Toxin
L1F17_RS23335 (L1F17_23335)	4839706..4840128	+	423	WP_033632013.1	helix-turn-helix domain-containing protein	Antitoxin
L1F17_RS23340 (L1F17_23340)	4840132..4842048	-	1917	WP_015379179.1	PRD domain-containing protein	-
L1F17_RS23345 (L1F17_23345)	4842100..4843314	-	1215	WP_033632011.1	lactonase family protein	-
L1F17_RS23350 (L1F17_23350)	4843402..4844142	-	741	WP_033648993.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12331.22 Da
• Isoelectric Point: 10.0957

>T232106 WP_060560355.1 NZ_CP090908:4839398-4839709 [Serratia marcescens]
MHIISREPFNNATKQYPNEATALEAIYKALRRVEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T232106 NZ_CP127255:19126-19500 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCTGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
ACGGTTGCTGGACCAGCATTACGGCCTTACGCTGGATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCGGGTATTTCACTGTGCGATGCAGTGAACTTTCTCGTTGAAAAATACACACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15833.20 Da
• Isoelectric Point: 5.2297

>AT232106 WP_033632013.1 NZ_CP090908:4839706-4840128 [Serratia marcescens]
MITDAIKAADALLRAVPLLQGSRAQQDYREALELVEYLLENDERHPLIDMLAKKIAEYEDNSAEFTAFNQRITQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT232106 NZ_CP127255:18672-19037 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACAACCGCCCCTGGTGGGGGCTACCCTGCACCGTGATGCC
CTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGTTCA
GCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGTGAA
CTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTAGGCTCCTGTGG
TTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Structures

Toxin

• AlphaFold DB: A0A0P0QJB7