Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4783346..4783974 Replicon chromosome
Accession NZ_CP090908
Organism Serratia marcescens strain Sys06

Toxin (Protein)


Gene name higB Uniprot ID A0A0N7JQB8
Locus tag L1F17_RS23025 Protein ID WP_060560346.1
Coordinates 4783346..4783636 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B4GQY7
Locus tag L1F17_RS23030 Protein ID WP_015379150.1
Coordinates 4783651..4783974 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1F17_RS23015 (L1F17_23015) 4779760..4781781 + 2022 WP_033632027.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
L1F17_RS23020 (L1F17_23020) 4781976..4783115 - 1140 WP_049189088.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
L1F17_RS23025 (L1F17_23025) 4783346..4783636 + 291 WP_060560346.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L1F17_RS23030 (L1F17_23030) 4783651..4783974 + 324 WP_015379150.1 HigA family addiction module antitoxin Antitoxin
L1F17_RS23035 (L1F17_23035) 4783967..4784479 + 513 WP_042785417.1 M48 family metallopeptidase -
L1F17_RS23040 (L1F17_23040) 4784529..4785509 + 981 WP_060560347.1 Gfo/Idh/MocA family oxidoreductase -
L1F17_RS23045 (L1F17_23045) 4785511..4786242 + 732 WP_060560348.1 glutamine amidotransferase -
L1F17_RS23050 (L1F17_23050) 4786495..4787463 + 969 WP_033632025.1 TerC family protein -
L1F17_RS23055 (L1F17_23055) 4787726..4788961 + 1236 WP_004937146.1 serine/threonine transporter SstT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-94)

Antitoxin

Cro/C1-type helix-turn-helix domain

(24-71)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11596.29 Da        Isoelectric Point: 10.2325

>T232105 WP_060560346.1 NZ_CP090908:4783346-4783636 [Serratia marcescens]
MIKSFRDRYLEPFYLEGKRSRLIPGVLERQLARKLDILAAAQQERDLHHPTGNYYKRLSGPFQGWSSLRVNMHWRLMFQW
RHDAAEKIYLDPHQDT

Download         Length: 291 bp

>T232105 NZ_CP127254:83207-83512 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12141.78 Da        Isoelectric Point: 6.2285

>AT232105 WP_015379150.1 NZ_CP090908:4783651-4783974 [Serratia marcescens]
MLETTTREPTTVGEVLQEEYLTPLGIGQEELAKVLGVTRATVNRLCNGHRRMSVAEANLFADLFETTADFWLNLQAAHDR
WEARQADAQRVRLRPIKEILGRNITHA

Download         Length: 324 bp

>AT232105 NZ_CP127254:82987-83205 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGATTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0N7JQB8

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4GQY7

References