Detailed information of TA system TA023201

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1476984..1477355
• Replicon: chromosome
• Accession: NC_011415
• Organism: Escherichia coli SE11

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: ECSE_RS07285
• Protein ID: WP_001317028.1
• Coordinates: 1477161..1477355 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1476984..1477162 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSE_RS07260	1472939..1474312	+	1374	WP_000123738.1	ATP-dependent RNA helicase DbpA	-
ECSE_RS07265	1474441..1475376	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
ECSE_RS26695	1475428..1476663	-	1236	WP_000040852.1	site-specific integrase	-
ECSE_RS07275	1476665..1476880	-	216	WP_000079604.1	excisionase XisR	-
ECSE_RS07280	1476959..1477168	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1476984..1477162	+	179	NuclAT_0	-	Antitoxin
-	1476984..1477162	+	179	NuclAT_0	-	Antitoxin
-	1476984..1477162	+	179	NuclAT_0	-	Antitoxin
-	1476984..1477162	+	179	NuclAT_0	-	Antitoxin
ECSE_RS07285	1477161..1477355	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
ECSE_RS07290	1477412..1478221	-	810	WP_000166319.1	recombination protein RecT	-
ECSE_RS07295	1478214..1480814	-	2601	WP_000105146.1	exodeoxyribonuclease VIII	-
ECSE_RS07300	1480916..1481191	-	276	WP_012565066.1	protein RacC	-
ECSE_RS07305	1481266..1481436	-	171	WP_001352098.1	conserved protein, Rac prophage	-
ECSE_RS07310	1481436..1481657	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1475428..1526058	50630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T23201 WP_001317028.1 NC_011415:c1477355-1477161 [Escherichia coli SE11]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T23201 NC_011415:c1477355-1477161 [Escherichia coli SE11]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT23201 NC_011415:1476984-1477162 [Escherichia coli SE11]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin