Detailed information of TA system TA231947

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 71707..71960
• Replicon: plasmid plas1
• Accession: NZ_CP090835
• Organism: Klebsiella pneumoniae strain K-28

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: L0U94_RS26840
• Protein ID: WP_001312851.1
• Coordinates: 71707..71856 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	71901..71960 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0U94_RS26810 (67729)	67729..68130	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
L0U94_RS26815 (68063)	68063..68320	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
L0U94_RS26820 (68413)	68413..69066	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
L0U94_RS26825 (70005)	70005..70862	-	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-
L0U94_RS26830 (70855)	70855..70929	-	75	WP_001365705.1	RepA leader peptide Tap	-
L0U94_RS26835 (71174)	71174..71422	-	249	WP_000083839.1	replication regulatory protein RepA	-
L0U94_RS26840 (71707)	71707..71856	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (71901)	71901..71960	+	60	NuclAT_0	-	Antitoxin
- (71901)	71901..71960	+	60	NuclAT_0	-	Antitoxin
- (71901)	71901..71960	+	60	NuclAT_0	-	Antitoxin
- (71901)	71901..71960	+	60	NuclAT_0	-	Antitoxin
L0U94_RS26845 (72103)	72103..72576	-	474	WP_016240489.1	hypothetical protein	-
L0U94_RS26850 (72731)	72731..73321	-	591	WP_033807967.1	DUF2726 domain-containing protein	-
L0U94_RS26855 (73359)	73359..73568	-	210	WP_001298565.1	hemolysin expression modulator Hha	-
L0U94_RS26860 (73614)	73614..74075	-	462	WP_001233838.1	thermonuclease family protein	-
L0U94_RS26865 (74320)	74320..74532	-	213	WP_001309245.1	ANR family transcriptional regulator	-
L0U94_RS26870 (74661)	74661..75221	-	561	WP_000139321.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2 / mph(A) / sul1 / qacE / dfrA25 / blaCTX-M-3 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B	-	1..75446	75446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T231947 WP_001312851.1 NZ_CP090835:c71856-71707 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T231947 NZ_CP127021:1663402-1663863 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCTTACAATTATGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTGCAATTGAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGGACTC
ACTACGGCGAATACTCAATTACATTTGAGCCATTGAGAGTTTTTAAATATAAAGAAATATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT231947 NZ_CP090835:71901-71960 [Klebsiella pneumoniae]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin