Detailed information of TA system TA023192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 795168..795814
• Replicon: chromosome
• Accession: NC_011386
• Organism: Oligotropha carboxidovorans OM5

Toxin (Protein)

• Gene name: graT
• Uniprot ID: B6JDM4
• Locus tag: OCAR_RS03870
• Protein ID: WP_012561984.1
• Coordinates: 795533..795814 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OCAR_RS03865
• Protein ID: WP_012561983.1
• Coordinates: 795168..795524 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCAR_RS03830	791876..792259	+	384	WP_012561975.1	DUF302 domain-containing protein	-
OCAR_RS03835	792280..792543	+	264	WP_013913378.1	SHOCT domain-containing protein	-
OCAR_RS03840	792579..792827	+	249	WP_012561977.1	hypothetical protein	-
OCAR_RS03845	793160..793696	+	537	WP_012561978.1	hypothetical protein	-
OCAR_RS03850	793693..794010	+	318	WP_013913377.1	hypothetical protein	-
OCAR_RS03855	794007..794222	+	216	WP_012561980.1	hypothetical protein	-
OCAR_RS03860	794219..794770	+	552	WP_012561981.1	hypothetical protein	-
OCAR_RS03865	795168..795524	-	357	WP_012561983.1	HigA family addiction module antidote protein	Antitoxin
OCAR_RS03870	795533..795814	-	282	WP_012561984.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OCAR_RS03875	796163..797530	+	1368	WP_012561986.1	site-specific DNA-methyltransferase	-
OCAR_RS17895	797532..798608	+	1077	WP_012561987.1	DNA cytosine methyltransferase	-
OCAR_RS03890	798626..798856	-	231	WP_012561988.1	hexameric tyrosine-coordinated heme protein	-
OCAR_RS03895	798982..799701	-	720	WP_013913375.1	DUF3489 domain-containing protein	-
OCAR_RS03900	799806..800024	-	219	WP_012561990.1	hypothetical protein	-
OCAR_RS03905	800213..800797	+	585	WP_013913374.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	762268..833574	71306

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (25-111)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10592.14 Da
• Isoelectric Point: 10.1526

>T23192 WP_012561984.1 NC_011386:c795814-795533 [Afipia carboxidovorans OM5]
VIKSFGDKRTAAIFAGYAVRGLPPPIQERARAKLLVIDAAKQLDDLRQPPGNRLEALAGHRKGQHSIRINDQWRICFVWR
NGETFDVEIVDYH

Nucleotide

• Length: 282 bp

>T23192 NC_011386:c795814-795533 [Afipia carboxidovorans OM5]
GTGATCAAGAGCTTCGGCGATAAACGCACCGCCGCAATCTTCGCAGGCTACGCCGTGCGCGGTTTGCCGCCGCCGATTCA
AGAGCGGGCTCGGGCGAAGCTGTTGGTGATCGACGCAGCAAAGCAACTCGACGATCTGCGCCAGCCGCCAGGCAACAGAC
TGGAGGCGTTGGCGGGTCACCGTAAGGGCCAGCACAGTATCCGCATCAACGATCAATGGCGGATATGCTTTGTGTGGCGG
AATGGCGAAACCTTCGATGTCGAAATTGTCGACTATCATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13565.63 Da
• Isoelectric Point: 10.5936

>AT23192 WP_012561983.1 NC_011386:c795524-795168 [Afipia carboxidovorans OM5]
MMSIKRENIDKGEIDFADVRSGRRLPPIHPGTILRDEFLTPMQISVYELASAIKTPRSRINDIVLGRRTITTDTALRLGR
YLGTSPEFWINLQARYDLDVANRTVRRKIEQEITPRAA

Nucleotide

• Length: 357 bp

>AT23192 NC_011386:c795524-795168 [Afipia carboxidovorans OM5]
ATGATGAGCATCAAGCGTGAAAACATCGACAAGGGAGAGATCGACTTCGCCGACGTGCGTTCCGGGCGACGGCTGCCGCC
AATCCATCCGGGCACGATCCTCCGCGATGAGTTCCTGACCCCTATGCAGATCAGCGTCTATGAGCTTGCCAGCGCAATCA
AGACGCCACGTTCGCGGATCAACGACATCGTACTGGGGCGGCGCACGATTACCACCGATACCGCACTACGGCTCGGACGC
TATCTTGGAACCTCGCCGGAGTTCTGGATCAATCTGCAGGCGCGCTACGATCTCGATGTCGCCAATCGCACCGTTCGCCG
TAAGATCGAACAGGAGATCACGCCACGTGCAGCTTGA

Structures

Toxin

• AlphaFold DB: B6JDM4