Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1195275..1196002 Replicon chromosome
Accession NZ_CP090796
Organism Enterobacter hormaechei strain Y-2

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3V067
Locus tag L0Y15_RS05605 Protein ID WP_022650547.1
Coordinates 1195691..1196002 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B3V464
Locus tag L0Y15_RS05600 Protein ID WP_022650546.1
Coordinates 1195275..1195694 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0Y15_RS05585 (L0Y15_05585) 1192347..1193468 - 1122 WP_003858868.1 YbdK family carboxylate-amine ligase -
L0Y15_RS05590 (L0Y15_05590) 1193574..1194464 + 891 WP_022650544.1 oxidoreductase -
L0Y15_RS05595 (L0Y15_05595) 1194517..1194951 - 435 WP_022650545.1 hypothetical protein -
L0Y15_RS05600 (L0Y15_05600) 1195275..1195694 - 420 WP_022650546.1 helix-turn-helix domain-containing protein Antitoxin
L0Y15_RS05605 (L0Y15_05605) 1195691..1196002 - 312 WP_022650547.1 type II toxin-antitoxin system HigB family toxin Toxin
L0Y15_RS05610 (L0Y15_05610) 1196458..1197114 + 657 WP_022650548.1 hypothetical protein -
L0Y15_RS05615 (L0Y15_05615) 1197227..1197682 - 456 WP_022650549.1 hypothetical protein -
L0Y15_RS05620 (L0Y15_05620) 1197965..1198846 - 882 WP_022650550.1 LysR family transcriptional regulator -
L0Y15_RS05625 (L0Y15_05625) 1198952..1199731 + 780 WP_003858848.1 acetolactate decarboxylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12554.34 Da        Isoelectric Point: 9.9435

>T231917 WP_022650547.1 NZ_CP090796:c1196002-1195691 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Download         Length: 312 bp

>T231917 NZ_CP126994:2600236-2600562 [Acidisoma sp. PAMC 29798]
ATAGTGCGGGGCGACTTGCTGACAGTGGCCGTCCACGGCGATTTCGGAAAACATCGCCCCGCATTGGTCATCCAAGCCGA
TCAGTTCAGCGAGCACACCACCGTCACCGTGTTGCCGCTGTCGAGCAGCATGGTCGATGCGCCCTTGCTCCGCATCACTG
TGCAGCCCAGCACTGAGAACGGTCTGCGAGCACCGTCCCAGGTGATGATCGACAAGGCCACGACGATTAAGCGCGAGAAG
GCGGGGTCGGCCTTCGGTCGCATCGAAACCGCGACGTTGGTAGAGATCGAGCGATGTTTGGCCGTCTTTCTGGGCATCGC
GAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15447.51 Da        Isoelectric Point: 4.6051

>AT231917 WP_022650546.1 NZ_CP090796:c1195694-1195275 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp

>AT231917 NZ_CP126994:2600015-2600239 [Acidisoma sp. PAMC 29798]
ATGGCAACGCCTCCCGGGAATGCCCGCGTTCAGAAGCACCGCGACGCGTTGCGCCACGCGGGTCTGCGACCGGTGCAGAT
TTGGGTGCCGGATACGCGGCGGCCGGAGTTCGCGGCGGAATGTCGGCGGCAATCCGTTCTCGTGGCAGAAGCCGATCGGT
CTGATATGGGTTTGCGCCAGTTCATGGATGAGGCCATCGCGGATCTTGACGGCTGGACCGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3V067

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3V464

References