Detailed information of TA system TA231897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1337432..1338159
• Replicon: chromosome
• Accession: NZ_CP090792
• Organism: Enterobacter hormaechei strain FY-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B3V067
• Locus tag: L0Y16_RS06435
• Protein ID: WP_022650547.1
• Coordinates: 1337848..1338159 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L0Y16_RS06430
• Protein ID: WP_032652900.1
• Coordinates: 1337432..1337851 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y16_RS06415 (L0Y16_06415)	1334503..1335624	-	1122	WP_032652893.1	YbdK family carboxylate-amine ligase	-
L0Y16_RS06420 (L0Y16_06420)	1335730..1336620	+	891	WP_032652895.1	oxidoreductase	-
L0Y16_RS06425 (L0Y16_06425)	1336674..1337108	-	435	WP_032652897.1	hypothetical protein	-
L0Y16_RS06430 (L0Y16_06430)	1337432..1337851	-	420	WP_032652900.1	helix-turn-helix domain-containing protein	Antitoxin
L0Y16_RS06435 (L0Y16_06435)	1337848..1338159	-	312	WP_022650547.1	type II toxin-antitoxin system HigB family toxin	Toxin
L0Y16_RS06440 (L0Y16_06440)	1338615..1339271	+	657	WP_080473509.1	hypothetical protein	-
L0Y16_RS06445 (L0Y16_06445)	1339385..1339840	-	456	WP_180244858.1	hypothetical protein	-
L0Y16_RS06450 (L0Y16_06450)	1340131..1341012	-	882	WP_003858850.1	LysR family transcriptional regulator	-
L0Y16_RS06455 (L0Y16_06455)	1341118..1341897	+	780	WP_026094386.1	acetolactate decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12554.34 Da
• Isoelectric Point: 9.9435

>T231897 WP_022650547.1 NZ_CP090792:c1338159-1337848 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Nucleotide

• Length: 312 bp

>T231897 NZ_CP126987:c5226439-5226056 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15437.48 Da
• Isoelectric Point: 4.6051

>AT231897 WP_032652900.1 NZ_CP090792:c1337851-1337432 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESSEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Nucleotide

• Length: 420 bp

>AT231897 NZ_CP126987:c5226681-5226439 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A8B3V067