Detailed information of TA system TA023181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2963429..2964005
• Replicon: chromosome
• Accession: NC_011365
• Organism: Gluconacetobacter diazotrophicus PAl 5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A9H9Y8
• Locus tag: GDIA_RS13540
• Protein ID: WP_012228624.1
• Coordinates: 2963724..2964005 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GDIA_RS13535
• Protein ID: WP_012228623.1
• Coordinates: 2963429..2963707 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDIA_RS13520	2958525..2959529	+	1005	WP_012228618.1	phosphate ABC transporter substrate-binding protein PstS	-
GDIA_RS13525	2960049..2961806	+	1758	WP_012554501.1	hypothetical protein	-
GDIA_RS13530	2962016..2963371	+	1356	WP_012554502.1	inorganic phosphate transporter	-
GDIA_RS13535	2963429..2963707	-	279	WP_012228623.1	HigA family addiction module antidote protein	Antitoxin
GDIA_RS13540	2963724..2964005	-	282	WP_012228624.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GDIA_RS13545	2964141..2964965	+	825	WP_012553437.1	IS5-like element ISGdi2 family transposase	-
GDIA_RS13550	2964982..2965707	-	726	WP_012228625.1	amino acid ABC transporter ATP-binding protein	-
GDIA_RS13555	2965704..2967257	-	1554	WP_012554504.1	ABC transporter substrate-binding protein/permease	-
GDIA_RS13560	2967483..2968223	+	741	WP_012228627.1	class II aldolase/adducin family protein	-
GDIA_RS13565	2968231..2968659	-	429	WP_012228628.1	metalloregulator ArsR/SmtB family transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2964168..2964965	797

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10609.15 Da
• Isoelectric Point: 8.9890

>T23181 WP_012228624.1 NC_011365:c2964005-2963724 [Gluconacetobacter diazotrophicus PA1 5]
MIRTFADKRAEQVFTGKLPKGFQAQIANAARPKLRMLAAATVLDDLRVPPANRLEALAGDRQGQYSIRINDQFRICFVWR
DGDAYDVEIIDYH

Nucleotide

• Length: 282 bp

>T23181 NC_011365:c2964005-2963724 [Gluconacetobacter diazotrophicus PA1 5]
ATGATTCGAACCTTTGCCGACAAGCGAGCGGAACAGGTTTTTACCGGGAAGCTGCCAAAAGGCTTTCAGGCGCAGATCGC
CAATGCGGCACGACCGAAACTGCGCATGCTGGCAGCGGCGACCGTCCTCGACGATCTGCGGGTCCCGCCGGCAAACCGCC
TTGAGGCACTGGCTGGCGACCGGCAGGGGCAATACTCGATCCGCATCAACGACCAGTTCCGGATTTGCTTCGTATGGCGT
GACGGCGACGCCTATGATGTCGAAATCATCGATTATCACTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10217.63 Da
• Isoelectric Point: 5.7012

>AT23181 WP_012228623.1 NC_011365:c2963707-2963429 [Gluconacetobacter diazotrophicus PA1 5]
MTELHNVSAGDVLREEFMLPFGLSARALAAEIGVPANRLSDIVRGRRSVTADTAILLAERFGTSAEFWMNLQVAYDLEEA
REKLASRHRSTL

Nucleotide

• Length: 279 bp

>AT23181 NC_011365:c2963707-2963429 [Gluconacetobacter diazotrophicus PA1 5]
ATGACCGAACTTCACAACGTATCGGCGGGCGACGTGCTGCGGGAGGAATTCATGCTCCCGTTCGGCCTGTCCGCCCGCGC
CCTGGCGGCGGAAATCGGCGTGCCGGCCAACCGCCTGAGCGATATCGTGCGGGGGCGGCGGAGCGTGACGGCGGATACGG
CCATCCTGCTGGCGGAACGGTTTGGTACATCCGCCGAATTCTGGATGAACCTCCAGGTGGCCTACGACCTTGAAGAAGCA
CGGGAGAAGCTGGCAAGCCGGCACCGCTCCACCCTGTAA

Structures

Toxin

• AlphaFold DB: A9H9Y8