Detailed information of TA system TA023171

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 5510915..5511327
• Replicon: chromosome
• Accession: NC_011353
• Organism: Escherichia coli O157:H7 str. EC4115

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: ECH74115_RS29045
• Protein ID: WP_000132630.1
• Coordinates: 5510915..5511256 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	5511251..5511327 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECH74115_RS34640	5506483..5506683	+	201	WP_001310455.1	hypothetical protein	-
ECH74115_RS29025	5506926..5508338	-	1413	WP_000199295.1	PLP-dependent aminotransferase family protein	-
ECH74115_RS29030	5508515..5508679	+	165	WP_000394274.1	DUF1127 domain-containing protein	-
ECH74115_RS29035	5508776..5509657	+	882	WP_001304007.1	DUF262 domain-containing protein	-
ECH74115_RS32300	5509705..5510868	+	1164	WP_001304006.1	DUF1524 domain-containing protein	-
ECH74115_RS29045	5510915..5511256	-	342	WP_000132630.1	endoribonuclease SymE	Toxin
-	5511251..5511327	+	77	NuclAT_14	-	Antitoxin
-	5511251..5511327	+	77	NuclAT_14	-	Antitoxin
-	5511251..5511327	+	77	NuclAT_14	-	Antitoxin
-	5511251..5511327	+	77	NuclAT_14	-	Antitoxin
-	5511251..5511327	+	77	NuclAT_15	-	Antitoxin
-	5511251..5511327	+	77	NuclAT_15	-	Antitoxin
-	5511251..5511327	+	77	NuclAT_15	-	Antitoxin
-	5511251..5511327	+	77	NuclAT_15	-	Antitoxin
ECH74115_RS29050	5511477..5513231	-	1755	WP_000110082.1	restriction endonuclease subunit S	-
ECH74115_RS29055	5513231..5514700	-	1470	WP_001302468.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T23171 WP_000132630.1 NC_011353:c5511256-5510915 [Escherichia coli O157:H7 str. EC4115]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T23171 NC_011353:c5511256-5510915 [Escherichia coli O157:H7 str. EC4115]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT23171 NC_011353:5511251-5511327 [Escherichia coli O157:H7 str. EC4115]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin