Detailed information of TA system TA023157

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2923474..2923699
• Replicon: chromosome
• Accession: NC_011353
• Organism: Escherichia coli O157:H7 str. EC4115

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: ECH74115_RS15935
• Protein ID: WP_000813254.1
• Coordinates: 2923474..2923629 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2923641..2923699 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECH74115_RS15900	2918759..2919817	-	1059	WP_000483509.1	site-specific DNA-methyltransferase	-
ECH74115_RS15905	2919968..2920165	-	198	WP_000917735.1	hypothetical protein	-
ECH74115_RS15910	2920392..2921213	-	822	WP_000762902.1	antitermination protein	-
ECH74115_RS15915	2921210..2921584	-	375	WP_000904171.1	RusA family crossover junction endodeoxyribonuclease	-
ECH74115_RS15920	2921597..2922646	-	1050	WP_001265156.1	DUF968 domain-containing protein	-
ECH74115_RS32960	2922648..2922926	-	279	WP_012779355.1	hypothetical protein	-
ECH74115_RS15930	2922996..2923253	-	258	WP_001217394.1	hypothetical protein	-
ECH74115_RS15935	2923474..2923629	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2923641..2923699	+	59	-	-	Antitoxin
ECH74115_RS32965	2923875..2923979	-	105	WP_001278450.1	hypothetical protein	-
ECH74115_RS15940	2924095..2924457	-	363	WP_000610377.1	DUF551 domain-containing protein	-
ECH74115_RS15945	2924454..2924825	-	372	WP_000137941.1	hypothetical protein	-
ECH74115_RS15950	2924861..2925073	-	213	WP_000063625.1	hypothetical protein	-
ECH74115_RS15955	2925122..2925478	-	357	WP_001302146.1	hypothetical protein	-
ECH74115_RS15960	2925535..2925930	-	396	WP_001118161.1	DUF977 family protein	-
ECH74115_RS15965	2925946..2926716	-	771	WP_000537576.1	DUF1627 domain-containing protein	-
ECH74115_RS34070	2926751..2927293	-	543	WP_157837342.1	replication protein	-
ECH74115_RS34075	2927205..2928245	-	1041	WP_000020570.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ospG	2892265..2985110	92845

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T23157 WP_000813254.1 NC_011353:c2923629-2923474 [Escherichia coli O157:H7 str. EC4115]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T23157 NC_011353:c2923629-2923474 [Escherichia coli O157:H7 str. EC4115]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT23157 NC_011353:2923641-2923699 [Escherichia coli O157:H7 str. EC4115]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin