Detailed information of TA system TA231569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1519909..1520504
• Replicon: chromosome
• Accession: NZ_CP090648
• Organism: Pseudomonas aeruginosa strain PA1616

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: L0025_RS07205
• Protein ID: WP_003113526.1
• Coordinates: 1520226..1520504 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L0025_RS07200
• Protein ID: WP_003113527.1
• Coordinates: 1519909..1520214 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0025_RS07180 (L0025_07180)	1515985..1516242	+	258	WP_010793759.1	ATP-binding protein	-
L0025_RS07185 (L0025_07185)	1516618..1517481	-	864	WP_010793758.1	integrase domain-containing protein	-
L0025_RS07190 (L0025_07190)	1518083..1519225	-	1143	WP_010793757.1	STY4528 family pathogenicity island replication protein	-
L0025_RS07200 (L0025_07200)	1519909..1520214	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
L0025_RS07205 (L0025_07205)	1520226..1520504	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0025_RS07210 (L0025_07210)	1520557..1520685	-	129	Protein_1421	integrase	-
L0025_RS07215 (L0025_07215)	1520833..1523061	+	2229	WP_003099265.1	TonB-dependent receptor	-
L0025_RS07220 (L0025_07220)	1523131..1523778	-	648	WP_003095021.1	carbonate dehydratase	-
L0025_RS07225 (L0025_07225)	1523840..1525078	-	1239	WP_010793756.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T231569 WP_003113526.1 NZ_CP090648:c1520504-1520226 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T231569 NZ_CP126931:335233-335760 [Escherichia coli O78:H51]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT231569 WP_003113527.1 NZ_CP090648:c1520214-1519909 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT231569 NZ_CP126931:334960-335226 [Escherichia coli O78:H51]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: V6ALY3