Detailed information of TA system TA231564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 731608..732203
• Replicon: chromosome
• Accession: NZ_CP090647
• Organism: Pseudomonas aeruginosa strain PA1681

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: L0024_RS03445
• Protein ID: WP_003113526.1
• Coordinates: 731608..731886 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L0024_RS03450
• Protein ID: WP_003113527.1
• Coordinates: 731898..732203 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0024_RS03425 (L0024_03425)	727034..728272	+	1239	WP_014603324.1	C69 family dipeptidase	-
L0024_RS03430 (L0024_03430)	728334..728981	+	648	WP_003095021.1	carbonate dehydratase	-
L0024_RS03435 (L0024_03435)	729051..731279	-	2229	WP_003113525.1	TonB-dependent receptor	-
L0024_RS03440 (L0024_03440)	731427..731555	+	129	Protein_681	integrase	-
L0024_RS03445 (L0024_03445)	731608..731886	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0024_RS03450 (L0024_03450)	731898..732203	+	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
L0024_RS03455 (L0024_03455)	732839..733414	+	576	WP_247354878.1	hypothetical protein	-
L0024_RS03460 (L0024_03460)	733418..734332	+	915	WP_247354880.1	DUF4868 domain-containing protein	-
L0024_RS03465 (L0024_03465)	734390..734725	-	336	WP_247354882.1	hypothetical protein	-
L0024_RS03470 (L0024_03470)	734781..735047	-	267	WP_023088595.1	hypothetical protein	-
L0024_RS03475 (L0024_03475)	735157..735429	+	273	WP_003115921.1	hypothetical protein	-
L0024_RS03480 (L0024_03480)	735422..735637	+	216	WP_235582466.1	hypothetical protein	-
L0024_RS03485 (L0024_03485)	735641..735928	+	288	WP_247354884.1	DUF5447 family protein	-
L0024_RS03490 (L0024_03490)	735936..736580	+	645	WP_070743358.1	DNA cytosine methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T231564 WP_003113526.1 NZ_CP090647:731608-731886 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T231564 NZ_CP126929:c164211-163924 [Escherichia coli O2:K1:H4]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT231564 WP_003113527.1 NZ_CP090647:731898-732203 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT231564 NZ_CP126929:c164459-164208 [Escherichia coli O2:K1:H4]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: V6ALY3