Detailed information of TA system TA023146

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2101953..2102178
• Replicon: chromosome
• Accession: NC_011353
• Organism: Escherichia coli O157:H7 str. EC4115

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: ECH74115_RS11270
• Protein ID: WP_000813258.1
• Coordinates: 2101953..2102108 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2102120..2102178 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECH74115_RS11220	2096956..2097387	-	432	WP_001302123.1	tellurite resistance protein	-
ECH74115_RS11235	2097838..2098551	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
ECH74115_RS33815	2098687..2098884	-	198	WP_000917763.1	hypothetical protein	-
ECH74115_RS11245	2099109..2099663	-	555	WP_000640035.1	DUF1133 family protein	-
ECH74115_RS11250	2099726..2100031	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
ECH74115_RS11255	2100044..2101093	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
ECH74115_RS11260	2101095..2101367	-	273	WP_000191872.1	hypothetical protein	-
ECH74115_RS11265	2101489..2101833	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
ECH74115_RS11270	2101953..2102108	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2102120..2102178	+	59	-	-	Antitoxin
ECH74115_RS11275	2102399..2102956	-	558	WP_000104474.1	DUF551 domain-containing protein	-
ECH74115_RS11280	2102958..2103176	-	219	WP_000683609.1	DUF4014 family protein	-
ECH74115_RS11285	2103304..2103615	-	312	WP_001289673.1	hypothetical protein	-
ECH74115_RS11290	2103608..2103835	-	228	WP_000699809.1	hypothetical protein	-
ECH74115_RS11295	2103832..2104113	-	282	WP_000603384.1	DNA-binding protein	-
ECH74115_RS11300	2104146..2104862	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
ECH74115_RS33820	2104896..2105357	-	462	WP_000139447.1	replication protein	-
ECH74115_RS33825	2105350..2106393	-	1044	WP_001262402.1	hypothetical protein	-
ECH74115_RS11315	2106462..2106887	-	426	WP_000693878.1	Rha family transcriptional regulator	-
ECH74115_RS11320	2106871..2107113	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2075432..2163590	88158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T23146 WP_000813258.1 NC_011353:c2102108-2101953 [Escherichia coli O157:H7 str. EC4115]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T23146 NC_011353:c2102108-2101953 [Escherichia coli O157:H7 str. EC4115]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT23146 NC_011353:2102120-2102178 [Escherichia coli O157:H7 str. EC4115]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin