Detailed information of TA system TA023143

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1748700..1748925
• Replicon: chromosome
• Accession: NC_011353
• Organism: Escherichia coli O157:H7 str. EC4115

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: ECH74115_RS09520
• Protein ID: WP_000813254.1
• Coordinates: 1748770..1748925 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1748700..1748758 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECH74115_RS33735	1744154..1745194	+	1041	WP_000020570.1	DNA-binding protein	-
ECH74115_RS33740	1745106..1745648	+	543	WP_157837342.1	replication protein	-
ECH74115_RS09490	1745683..1746453	+	771	WP_000537576.1	DUF1627 domain-containing protein	-
ECH74115_RS09495	1746469..1746864	+	396	WP_001118161.1	DUF977 family protein	-
ECH74115_RS09500	1746921..1747277	+	357	WP_001302146.1	hypothetical protein	-
ECH74115_RS09505	1747326..1747538	+	213	WP_000063625.1	hypothetical protein	-
ECH74115_RS09510	1747574..1747945	+	372	WP_000137941.1	hypothetical protein	-
ECH74115_RS09515	1747942..1748304	+	363	WP_000610377.1	DUF551 domain-containing protein	-
ECH74115_RS32690	1748420..1748524	+	105	WP_001278450.1	hypothetical protein	-
-	1748700..1748758	-	59	-	-	Antitoxin
ECH74115_RS09520	1748770..1748925	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
ECH74115_RS09525	1749146..1749403	+	258	WP_001217394.1	hypothetical protein	-
ECH74115_RS32695	1749473..1749751	+	279	WP_012779355.1	hypothetical protein	-
ECH74115_RS09535	1749753..1750802	+	1050	WP_001265156.1	DUF968 domain-containing protein	-
ECH74115_RS09540	1750815..1751189	+	375	WP_000904171.1	RusA family crossover junction endodeoxyribonuclease	-
ECH74115_RS09545	1751186..1752007	+	822	WP_000762902.1	antitermination protein	-
ECH74115_RS09550	1752234..1752431	+	198	WP_000917735.1	hypothetical protein	-
ECH74115_RS09555	1752582..1753640	+	1059	WP_000483509.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T23143 WP_000813254.1 NC_011353:1748770-1748925 [Escherichia coli O157:H7 str. EC4115]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T23143 NC_011353:1748770-1748925 [Escherichia coli O157:H7 str. EC4115]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT23143 NC_011353:c1748758-1748700 [Escherichia coli O157:H7 str. EC4115]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin