Detailed information of TA system TA231428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4225676..4226278
• Replicon: chromosome
• Accession: NZ_CP090611
• Organism: Escherichia coli isolate DCE7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LZG50_RS20755
• Protein ID: WP_000897305.1
• Coordinates: 4225967..4226278 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LZG50_RS20750
• Protein ID: WP_000356397.1
• Coordinates: 4225676..4225966 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZG50_RS20730 (4222179)	4222179..4223081	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LZG50_RS20735 (4223078)	4223078..4223713	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LZG50_RS20740 (4223710)	4223710..4224639	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
LZG50_RS20745 (4224855)	4224855..4225073	-	219	WP_001346587.1	CopG family transcriptional regulator	-
LZG50_RS20750 (4225676)	4225676..4225966	-	291	WP_000356397.1	NadS family protein	Antitoxin
LZG50_RS20755 (4225967)	4225967..4226278	-	312	WP_000897305.1	hypothetical protein	Toxin
LZG50_RS20760 (4226507)	4226507..4227415	+	909	WP_001318161.1	alpha/beta hydrolase	-
LZG50_RS20765 (4227479)	4227479..4228420	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LZG50_RS20770 (4228465)	4228465..4228902	-	438	WP_000560978.1	D-aminoacyl-tRNA deacylase	-
LZG50_RS20775 (4228899)	4228899..4229771	-	873	WP_000920762.1	virulence factor BrkB family protein	-
LZG50_RS20780 (4229765)	4229765..4230364	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T231428 WP_000897305.1 NZ_CP090611:c4226278-4225967 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T231428 NZ_CP126446:259360-259710 [Pontibacillus chungwhensis]
TTGATCGTAAAAAGAGGCGACGTTTACTTTGCTGACCTTTCCCCGGTCGTTGGCTCAGAACAGGGAGGCGTCCGCCCAGT
CCTCATCCTTCAAAATGATATTGGTAATCGCTTCAGCCCCACAGTCATTGTGGCGGCTATTACTGCGCAAATACAAAAAG
CAAAATTACCCACACACGTAGAAATTGATGCTGAACGATATGGATTCGAGCGAGACTCTGTCATATTGTTAGAGCAGATC
CGTACAATTGATAAGCAAAGACTTACAGACAAAATTACCCATCTAGATGAAGCTATGATGAGCAGGGTTGATGAAGCATT
ACAAATCAGTTTAGGGCTCATTGACTTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT231428 WP_000356397.1 NZ_CP090611:c4225966-4225676 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT231428 NZ_CP126446:259074-259355 [Pontibacillus chungwhensis]
ATTTTAGTGTCCGATAGTTCACAAGAAATTGTAGTGAAATTACCACAGCATTTATTAAATGAACTTGATGGTATGGTACA
ACTCGAAAATGGTGATCGGAGCGATTTTATTTACCAGGCAACGAAAATGTATTTGCGGGAGCGTAAAAAACGCCACATTC
GTGAGTCGATGCGTCAGGGCTACATGGAAATGGCAAAGATCAATCTAAACATTGCTTCAGAAGCTTTTCAAGCTGAAGAG
GAGGCAGAAACTACCTGCGAACGCCTAGTGAGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7