Detailed information of TA system TA231420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2022371..2022933
• Replicon: chromosome
• Accession: NZ_CP090611
• Organism: Escherichia coli isolate DCE7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A786YE86
• Locus tag: LZG50_RS09800
• Protein ID: WP_000605674.1
• Coordinates: 2022371..2022649 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LZG50_RS09805
• Protein ID: WP_000781364.1
• Coordinates: 2022649..2022933 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZG50_RS09775 (2017958)	2017958..2018389	-	432	WP_000152307.1	peroxiredoxin OsmC	-
LZG50_RS09780 (2018734)	2018734..2018949	+	216	WP_000495766.1	biofilm-dependent modulation protein	-
LZG50_RS09785 (2019051)	2019051..2019188	+	138	WP_000841554.1	stationary-phase-induced ribosome-associated protein	-
LZG50_RS09790 (2019344)	2019344..2021041	+	1698	WP_000433462.1	malate dehydrogenase	-
LZG50_RS09795 (2021175)	2021175..2022185	+	1011	WP_052912873.1	alcohol dehydrogenase AdhP	-
LZG50_RS09800 (2022371)	2022371..2022649	+	279	WP_000605674.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZG50_RS09805 (2022649)	2022649..2022933	+	285	WP_000781364.1	HigA family addiction module antitoxin	Antitoxin
LZG50_RS09810 (2022984)	2022984..2023637	-	654	WP_000045647.1	formate dehydrogenase-N subunit gamma	-
LZG50_RS09815 (2023630)	2023630..2024514	-	885	WP_001240584.1	formate dehydrogenase N subunit beta	-
LZG50_RS09820 (2024527)	2024527..2027574	-	3048	WP_096890942.1	formate dehydrogenase-N subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10609.23 Da
• Isoelectric Point: 6.8588

>T231420 WP_000605674.1 NZ_CP090611:2022371-2022649 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDEIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T231420 NZ_CP126348:3357090-3357308 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10386.11 Da
• Isoelectric Point: 8.8567

>AT231420 WP_000781364.1 NZ_CP090611:2022649-2022933 [Escherichia coli]
MKMANHPRPGDIILESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Nucleotide

• Length: 285 bp

>AT231420 NZ_CP126348:3356690-3357064 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A786YE86