Detailed information of TA system TA023134

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1177960..1178185
• Replicon: chromosome
• Accession: NC_011353
• Organism: Escherichia coli O157:H7 str. EC4115

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: ECH74115_RS06175
• Protein ID: WP_000813263.1
• Coordinates: 1178030..1178185 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1177960..1178018 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECH74115_RS06150	1173235..1174326	+	1092	WP_001205823.1	hypothetical protein	-
ECH74115_RS06155	1174333..1175079	+	747	WP_000788745.1	ATP-binding protein	-
ECH74115_RS06160	1175101..1175871	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
ECH74115_RS06165	1175887..1176300	+	414	WP_001151233.1	DUF977 family protein	-
ECH74115_RS06170	1176652..1177425	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1177960..1178018	-	59	-	-	Antitoxin
ECH74115_RS06175	1178030..1178185	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
ECH74115_RS32510	1178353..1178631	+	279	WP_001341388.1	hypothetical protein	-
ECH74115_RS06185	1178633..1179682	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
ECH74115_RS06190	1179695..1180066	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
ECH74115_RS06195	1180056..1180427	+	372	WP_000090264.1	antitermination protein	-
ECH74115_RS06200	1180579..1181397	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
ECH74115_RS06205	1181684..1181880	+	197	Protein_1066	TrmB family transcriptional regulator	-
ECH74115_RS06210	1182018..1182731	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T23134 WP_000813263.1 NC_011353:1178030-1178185 [Escherichia coli O157:H7 str. EC4115]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T23134 NC_011353:1178030-1178185 [Escherichia coli O157:H7 str. EC4115]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT23134 NC_011353:c1178018-1177960 [Escherichia coli O157:H7 str. EC4115]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin