Detailed information of TA system TA023130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 292452..293146
• Replicon: chromosome
• Accession: NC_011353
• Organism: Escherichia coli O157:H7 str. EC4115

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: ECH74115_RS01910
• Protein ID: WP_001263495.1
• Coordinates: 292748..293146 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ECH74115_RS01905
• Protein ID: WP_000554757.1
• Coordinates: 292452..292745 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECH74115_RS33430	288163..288660	+	498	Protein_251	REP-associated tyrosine transposase RayT	-
ECH74115_RS01890	288805..290517	-	1713	Protein_252	flagellar biosynthesis protein FlhA	-
ECH74115_RS01895	290489..291274	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ECH74115_RS01900	291345..292400	+	1056	WP_001226186.1	DNA polymerase IV	-
ECH74115_RS01905	292452..292745	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ECH74115_RS01910	292748..293146	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ECH74115_RS01915	293156..293608	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
ECH74115_RS01920	293927..294130	+	204	Protein_258	RNA ligase RtcB family protein	-
ECH74115_RS01925	294129..294650	+	522	Protein_259	peptide chain release factor H	-
ECH74115_RS01930	294707..296164	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
ECH74115_RS01935	296425..296883	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	297479..297559	+	81	NuclAT_11	-	-
-	297479..297559	+	81	NuclAT_11	-	-
-	297479..297559	+	81	NuclAT_11	-	-
-	297479..297559	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T23130 WP_001263495.1 NC_011353:292748-293146 [Escherichia coli O157:H7 str. EC4115]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T23130 NC_011353:292748-293146 [Escherichia coli O157:H7 str. EC4115]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT23130 WP_000554757.1 NC_011353:292452-292745 [Escherichia coli O157:H7 str. EC4115]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT23130 NC_011353:292452-292745 [Escherichia coli O157:H7 str. EC4115]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6