Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 286489..287028 Replicon chromosome
Accession NC_011353
Organism Escherichia coli O157:H7 str. EC4115

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag ECH74115_RS01870 Protein ID WP_001303998.1
Coordinates 286489..286749 (-) Length 87 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID C3TP07
Locus tag ECH74115_RS01875 Protein ID WP_000729705.1
Coordinates 286768..287028 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECH74115_RS01855 284172..284750 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
ECH74115_RS01860 284855..285622 + 768 WP_001301698.1 class II glutamine amidotransferase -
ECH74115_RS01865 285593..286333 - 741 WP_001225679.1 murein L,D-transpeptidase -
ECH74115_RS01870 286489..286749 - 261 WP_001303998.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ECH74115_RS01875 286768..287028 - 261 WP_000729705.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ECH74115_RS01880 287214..287987 + 774 WP_000543891.1 C40 family peptidase -
ECH74115_RS33430 288163..288660 + 498 Protein_251 REP-associated tyrosine transposase RayT -
ECH74115_RS01890 288805..290517 - 1713 Protein_252 flagellar biosynthesis protein FlhA -
ECH74115_RS01895 290489..291274 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-85)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10026.71 Da        Isoelectric Point: 10.3188

>T23129 WP_001303998.1 NC_011353:c286749-286489 [Escherichia coli O157:H7 str. EC4115]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Download         Length: 261 bp

>T23129 NC_011353:c286749-286489 [Escherichia coli O157:H7 str. EC4115]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9377.74 Da        Isoelectric Point: 5.0758

>AT23129 WP_000729705.1 NC_011353:c287028-286768 [Escherichia coli O157:H7 str. EC4115]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Download         Length: 261 bp

>AT23129 NC_011353:c287028-286768 [Escherichia coli O157:H7 str. EC4115]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References