Detailed information of TA system TA023125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1062466..1063054
• Replicon: chromosome
• Accession: NC_011333
• Organism: Helicobacter pylori G27

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: M3P300
• Locus tag: HPG27_RS05070
• Protein ID: WP_000921941.1
• Coordinates: 1062466..1062747 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: B5Z829
• Locus tag: HPG27_RS05075
• Protein ID: WP_000065301.1
• Coordinates: 1062740..1063054 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPG27_RS05035	1057679..1057942	+	264	WP_000578855.1	hypothetical protein	-
HPG27_RS05040	1057939..1058237	+	299	Protein_969	hypothetical protein	-
HPG27_RS05045	1058237..1059178	+	942	WP_000625134.1	Flp pilus assembly complex ATPase component TadA	-
HPG27_RS05050	1059175..1059630	+	456	WP_000795414.1	hypothetical protein	-
HPG27_RS05055	1059627..1059917	+	291	WP_000159819.1	hypothetical protein	-
HPG27_RS05060	1059918..1060475	+	558	WP_001167793.1	hypothetical protein	-
HPG27_RS05065	1060476..1062203	+	1728	WP_001208197.1	type IV secretory system conjugative DNA transfer family protein	-
HPG27_RS05070	1062466..1062747	-	282	WP_000921941.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
HPG27_RS05075	1062740..1063054	-	315	WP_000065301.1	hypothetical protein	Antitoxin
HPG27_RS05080	1063336..1065246	+	1911	WP_001162744.1	relaxase/mobilization nuclease domain-containing protein	-
HPG27_RS05085	1065271..1066443	-	1173	WP_000753636.1	hypothetical protein	-
HPG27_RS05090	1066475..1066759	-	285	WP_000394653.1	hypothetical protein	-
HPG27_RS05095	1066842..1067498	-	657	WP_000587407.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10891.80 Da
• Isoelectric Point: 9.5726

>T23125 WP_000921941.1 NC_011333:c1062747-1062466 [Helicobacter pylori G27]
MLKVRTKKDFLKDFNKHILSGRITESDVTSVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T23125 NC_011333:c1062747-1062466 [Helicobacter pylori G27]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTACAGAAAGCGA
TGTTACAAGCGTTGTTGATTGTCTCAAAGAGCAAAAACCACTCCAACAAAAATATTGCGACCATGCCTTAAGTGGTAATC
TTAAAGGGCTGAGAGAATGCCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12237.68 Da
• Isoelectric Point: 5.6761

>AT23125 WP_000065301.1 NC_011333:c1063054-1062740 [Helicobacter pylori G27]
MSNIITDYSQYNEKQLHNFLNSIEKQLLKAEGDKNKAIKKIQECELQERMIRQVLAQKHSQEKEPTPSLLNTINSKDDPE
YDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT23125 NC_011333:c1063054-1062740 [Helicobacter pylori G27]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCCTAAACTCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACGAATGATCAGACAGG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACCAAGTTTGTTAAATACGATTAACTCAAAAGATGACCCAGAA
TACGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Toxin

• AlphaFold DB: M3P300

Antitoxin

• AlphaFold DB: B5Z829