Detailed information of TA system TA231247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 3730970..3731511
• Replicon: chromosome
• Accession: NZ_CP090570
• Organism: Escherichia coli strain BY63

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Q32JN3
• Locus tag: LZE53_RS18360
• Protein ID: WP_000615980.1
• Coordinates: 3731233..3731511 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: LZE53_RS18355
• Protein ID: WP_000729707.1
• Coordinates: 3730970..3731230 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZE53_RS18335 (3726636)	3726636..3727421	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
LZE53_RS18340 (3727393)	3727393..3729105	+	1713	Protein_3596	flagellar biosynthesis protein FlhA	-
LZE53_RS18345 (3729338)	3729338..3729835	-	498	WP_232579830.1	REP-associated tyrosine transposase RayT	-
LZE53_RS18350 (3730011)	3730011..3730784	-	774	WP_000543885.1	C40 family peptidase	-
LZE53_RS18355 (3730970)	3730970..3731230	+	261	WP_000729707.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
LZE53_RS18360 (3731233)	3731233..3731511	+	279	WP_000615980.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
LZE53_RS18365 (3731667)	3731667..3732407	+	741	WP_001225679.1	peptidoglycan meso-diaminopimelic acid protein amidase	-
LZE53_RS18370 (3732378)	3732378..3733145	-	768	WP_000333380.1	class II glutamine amidotransferase	-
LZE53_RS18375 (3733351)	3733351..3733929	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (5-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T231247 WP_000615980.1 NZ_CP090570:3731233-3731511 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T231247 NZ_CP126166:2447474-2447887 [Salmonella enterica subsp. enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9421.80 Da
• Isoelectric Point: 5.0758

>AT231247 WP_000729707.1 NZ_CP090570:3730970-3731230 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKTKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT231247 NZ_CP126166:2447227-2447493 [Salmonella enterica subsp. enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A090NH48