Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 699045..699772 Replicon chromosome
Accession NZ_CP090570
Organism Escherichia coli strain BY63

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag LZE53_RS03385 Protein ID WP_000550189.1
Coordinates 699045..699359 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LZE53_RS03390 Protein ID WP_000560266.1
Coordinates 699356..699772 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZE53_RS03365 (695203) 695203..696189 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
LZE53_RS03370 (696268) 696268..696960 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
LZE53_RS03375 (697037) 697037..697540 - 504 WP_096191244.1 M48 family metallopeptidase -
LZE53_RS03380 (697625) 697625..698761 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
LZE53_RS03385 (699045) 699045..699359 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
LZE53_RS03390 (699356) 699356..699772 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LZE53_RS03395 (699817) 699817..701835 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
LZE53_RS03400 (702261) 702261..704612 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T231232 WP_000550189.1 NZ_CP090570:699045-699359 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T231232 NZ_CP126147:c5111-4701 [Sagittula sp. MA-2]
GTGATCCACATCCTCGACACCAACATCATCTCCGCCGCGCGCCGGGCCGAACGCGCGCCGGACCTCTCCCGCTGGCTGAC
CGCGCAGGACGAGGCCACGCTGTTCCTCAGCGTCGTCACCATAGGCGAGATCGAGCGCGGCATCGCCCTGCAGGAGCCGA
AGAACCCGCCCTTCGCCCGCGACCTGCGGCACTGGATGGACGGCATCCTGCGGCTCTTCTCCGACCGGCTGCTCGACTTC
ACCGCCGAGGACGCCCGCCGCTGGGGCCGCCTGTCGGCCAGGCTGGGCCATCCCGGCGCCGACCTGATGATCGCAGCACA
GGCGCTGAACCGCGACGCCACCGTCGTCACCCGCAACACCGCCGATTTCGCGCCCACCGGCGCGCGGCTGCTGGACCCGC
TAGGCCTCTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT231232 WP_000560266.1 NZ_CP090570:699356-699772 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT231232 NZ_CP126147:c5359-5108 [Sagittula sp. MA-2]
ATGCTTTCGCTTCAGGACGCCAAGAACCGCTTCAGCGCGGTGGTGGAGGCCGCGCTTGCCGGCCATCCGCAGGCCGTGTC
GCGCCGGGGCAAACCCGCCGTGGTCGTCGTCTCCGCCGAGGAATATGCAAGGCTGGTGCACGCCGCACAGGCCAGCCGCG
GCCGCTTTGCCGATCACCTGATGGCCATGCCCGACCCCGGCGACACCCCCCTGCCCCGCGCCAGCGCCGTGCCGCGCGAC
GTCGAGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References