Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 654364..655163 Replicon chromosome
Accession NZ_CP090570
Organism Escherichia coli strain BY63

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag LZE53_RS03150 Protein ID WP_000347273.1
Coordinates 654364..654828 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LZE53_RS03155 Protein ID WP_001307405.1
Coordinates 654828..655163 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZE53_RS03120 (649365) 649365..649799 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LZE53_RS03125 (649817) 649817..650695 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LZE53_RS03130 (650685) 650685..651464 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
LZE53_RS03135 (651475) 651475..651948 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
LZE53_RS03140 (651971) 651971..653251 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
LZE53_RS03145 (653500) 653500..654309 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LZE53_RS03150 (654364) 654364..654828 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LZE53_RS03155 (654828) 654828..655163 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LZE53_RS03160 (655312) 655312..656883 - 1572 WP_001273753.1 galactarate dehydratase -
LZE53_RS03165 (657258) 657258..658592 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LZE53_RS03170 (658608) 658608..659378 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T231231 WP_000347273.1 NZ_CP090570:c654828-654364 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T231231 NZ_CP126144:240644-240994 [Neobacillus sp. 114]
TTGATTGTCAAGCGTGGTGACGTATATTTTGCGGACCTTTCCCCAGTTGTTGGTTCTGAACAAGGCGGCGTCCGCCCTGT
ACTAGTCATCCAAAACGACATCGGGAATCGGTTTAGTCCCACAGTGATTGTTGCAGCGATTACAGCTCAAATTCAGAAAG
CGAAGCTCCCTACTCATGTTGAAATTGATGCGAAGCGCTACGGATTTGAACGCGACTCAGTCATTCTGTTAGAGCAAATT
CGCACAATTGATAAGCAGCGGTTAACCGATAAAATTACCCATCTCGATGACGAAATGATGGAAAAAGTGGATGAAGCCTT
GCAGGTAAGTCTTGGTCTCATCGAATTTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT231231 WP_001307405.1 NZ_CP090570:c655163-654828 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT231231 NZ_CP126144:240358-240639 [Neobacillus sp. 114]
GTGTCTGAATCCAGCGCAACTACGGAAATCTTGGTAAAATTACCGCAGCATCTATTAACTGAATTAGATGGTTTCGTAAA
GCAGGAAAATGTAAACCGCAGTGAGTTTATCTACCAAGCAACAAAAATGTATTTACGCGAACGAAAAACGAGACAAATTC
GCGAATCCATGAGACGTGGATACATGGAAATGGCAAAGATTAATCTTAGGATTGCGTCAGAAGCATTTCAAGCAGAATAT
GAGGCAGAGCATACTGTTGAACGTCTTGTAAGTGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References