Detailed information of TA system TA231229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1303121..1303705
• Replicon: chromosome
• Accession: NZ_CP090569
• Organism: Candidatus Endoriftia persephone isolate Tica-EPR-9o50.N

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G2FD58
• Locus tag: L0Y14_RS05940
• Protein ID: WP_006474125.1
• Coordinates: 1303430..1303705 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L0Y14_RS05935
• Protein ID: WP_005966092.1
• Coordinates: 1303121..1303417 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y14_RS05905 (L0Y14_05905)	1298188..1298451	+	264	WP_251859273.1	hypothetical protein	-
L0Y14_RS05910 (L0Y14_05910)	1298448..1298780	+	333	WP_006474128.1	hypothetical protein	-
L0Y14_RS05915 (L0Y14_05915)	1298801..1299154	+	354	WP_006474127.1	hypothetical protein	-
L0Y14_RS05920 (L0Y14_05920)	1299151..1299753	+	603	WP_005966104.1	tyrosine-type recombinase/integrase	-
L0Y14_RS05925 (L0Y14_05925)	1299854..1300039	+	186	WP_005966100.1	hypothetical protein	-
L0Y14_RS05930 (L0Y14_05930)	1300118..1302853	+	2736	WP_251859274.1	toprim domain-containing protein	-
L0Y14_RS05935 (L0Y14_05935)	1303121..1303417	-	297	WP_005966092.1	HigA family addiction module antitoxin	Antitoxin
L0Y14_RS05940 (L0Y14_05940)	1303430..1303705	-	276	WP_006474125.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0Y14_RS05945 (L0Y14_05945)	1303785..1304084	+	300	WP_251859275.1	hypothetical protein	-
L0Y14_RS05950 (L0Y14_05950)	1304086..1304289	+	204	WP_040820569.1	hypothetical protein	-
L0Y14_RS05955 (L0Y14_05955)	1304569..1305669	+	1101	WP_006475873.1	Fic family protein	-
L0Y14_RS05960 (L0Y14_05960)	1305772..1306017	-	246	WP_251859276.1	antirestriction protein ArdA	-
L0Y14_RS05965 (L0Y14_05965)	1305988..1306353	+	366	WP_251859277.1	BRO family protein	-
L0Y14_RS05970 (L0Y14_05970)	1306355..1306558	+	204	WP_010088852.1	hypothetical protein	-
L0Y14_RS05975 (L0Y14_05975)	1306761..1307129	+	369	WP_251859278.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1280977..1311413	30436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-91)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-65)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10769.31 Da
• Isoelectric Point: 8.0146

>T231229 WP_006474125.1 NZ_CP090569:c1303705-1303430 [Candidatus Endoriftia persephone]
MIKSFKCKQTQALYEGQCPRQFRAFRAQAERKLQMLDCAVELADLRLPPGNRLEKLSDDRQGQYSIRINQQWRICFSWTD
APCDVEITDYH

Nucleotide

• Length: 276 bp

>T231229 NZ_CP126141:36548-36853 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11354.12 Da
• Isoelectric Point: 9.9716

>AT231229 WP_005966092.1 NZ_CP090569:c1303417-1303121 [Candidatus Endoriftia persephone]
MNQMRPIHPGEILREEYLEPLGMSANALATALRVSAPRINDVVRERRGITVDTALRLARYFNTTPMYWMNLQAAYELKAA
RRKQDEIEREVLPLQRAS

Nucleotide

• Length: 297 bp

>AT231229 NZ_CP126141:36328-36546 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA

Structures

Toxin

• AlphaFold DB: G2FD58