Detailed information of TA system TA231189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2314761..2315406
• Replicon: chromosome
• Accession: NZ_CP090556
• Organism: Actinobacillus suis strain D20_277

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0G4M5
• Locus tag: L0F67_RS11200
• Protein ID: WP_014991853.1
• Coordinates: 2315062..2315406 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L0F67_RS11195
• Protein ID: WP_014991852.1
• Coordinates: 2314761..2315057 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0F67_RS11170 (L0F67_11155)	2309940..2310943	-	1004	Protein_2153	Holliday junction branch migration DNA helicase RuvB	-
L0F67_RS11175 (L0F67_11160)	2310962..2311567	-	606	WP_014991848.1	Holliday junction branch migration protein RuvA	-
L0F67_RS11180 (L0F67_11165)	2311699..2312475	-	777	WP_254240696.1	spermidine/putrescine ABC transporter permease PotC	-
L0F67_RS11185 (L0F67_11170)	2312475..2313341	-	867	WP_014991850.1	spermidine/putrescine ABC transporter permease PotB	-
L0F67_RS11190 (L0F67_11175)	2313328..2314443	-	1116	WP_014991851.1	spermidine/putrescine ABC transporter ATP-binding protein PotA	-
L0F67_RS11195 (L0F67_11180)	2314761..2315057	-	297	WP_014991852.1	NadS family protein	Antitoxin
L0F67_RS11200 (L0F67_11185)	2315062..2315406	-	345	WP_014991853.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0F67_RS11205 (L0F67_11190)	2315611..2317628	+	2018	Protein_2160	DNA helicase Rep	-
L0F67_RS11210 (L0F67_11195)	2317717..2318469	-	753	WP_014991855.1	TatD family hydrolase	-
L0F67_RS11215 (L0F67_11200)	2318563..2319714	-	1152	WP_014991856.1	methionine adenosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (39-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-83)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13348.34 Da
• Isoelectric Point: 7.9875

>T231189 WP_014991853.1 NZ_CP090556:c2315406-2315062 [Actinobacillus suis]
MNSDEYLTFVEVGTFEEDRKQLMADDEYQLFQAYLLEHYELGDFISHTGGCQKIRWRLPENNKGKSAGVRIIYYVRSLTG
RIYLITMYGKSEKVNLNAREKAIMKKIISRLIGE

Nucleotide

• Length: 345 bp

>T231189 NZ_CP126116:243534-243884 [Metabacillus sp. CT-WN-B3]
TTGATTGTCAAACGCGGCGACGTTTATTTTGCTGATTTATCACCCGTTGTTGGCTCTGAGCAAGGGGGCGTCCGTCCAGT
GCTGATCATCCAGAATGATATCGGAAACAGGTTCAGTCCAACGGTCATCATAGCAGCTATTACTGCTCAAATTCAAAAGG
CAAAATTGCCAACTCATGTGGAAATTGACTCCAAGCGTTATGGGTTTGAACGTGATTCTGTTATACTGTTAGAGCAGATT
CGTACAATTGATAAACAGAGATTGACTGATAAGATTACACACTTAGATGATGAAATGATGGATAAAGTGGACGAAGCTCT
GCAAATAAGCTTAGGACTCATTGATTTTTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11129.00 Da
• Isoelectric Point: 10.4778

>AT231189 WP_014991852.1 NZ_CP090556:c2315057-2314761 [Actinobacillus suis]
MEKRLFERLVQGLEEMVAIEKGEVEVPSHRIHAHKIPNTKAIRTQHHLKQAEFAQLLGVSTNLVQSWEIGRRVPNGAARK
LLAIIEQQPNLVPLLQSL

Nucleotide

• Length: 297 bp

>AT231189 NZ_CP126116:243248-243529 [Metabacillus sp. CT-WN-B3]
GTGTCTGAATCCAGCGCAACAACAGAAATCTTAATTCGTTTACCGCAAGCATTAGTCTCGGAATTAGACGGTCTTGTCAA
ACAAGAGAATGGGAACCGAAGCGAGTTAATTTATCAAGCAACAAAGATGTATATCCGCGAGCGTAAGAAACGCCAAATAC
GCGAATCAATGAGACGTGGATATATGGAGATGGCTAAGATTAACTTAAACATTGCTTCCGAAGCATTTCAAGCTGAATCA
GAGGCTGACCACACCGTTGAACGCTTAGTAAGCGGGGGTTAA

Structures

Toxin

• AlphaFold DB: K0G4M5