Detailed information of TA system TA231185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 598945..599599
• Replicon: chromosome
• Accession: NZ_CP090556
• Organism: Actinobacillus suis strain D20_277

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0G6E5
• Locus tag: L0F67_RS03015
• Protein ID: WP_015674148.1
• Coordinates: 599237..599599 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L0F67_RS03010
• Protein ID: WP_015674147.1
• Coordinates: 598945..599253 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0F67_RS02985 (L0F67_02980)	594090..594656	-	567	WP_015674143.1	septal ring lytic transglycosylase RlpA family protein	-
L0F67_RS02990 (L0F67_02985)	594831..595955	-	1125	WP_015674144.1	rod shape-determining protein RodA	-
L0F67_RS02995 (L0F67_02990)	595948..597918	-	1971	WP_015674145.1	penicillin-binding protein 2	-
L0F67_RS03000 (L0F67_02995)	598001..598468	-	468	WP_005598965.1	23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH	-
L0F67_RS03005 (L0F67_03000)	598491..598805	-	315	WP_015674146.1	ribosome silencing factor	-
L0F67_RS03010 (L0F67_03005)	598945..599253	-	309	WP_015674147.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
L0F67_RS03015 (L0F67_03010)	599237..599599	-	363	WP_015674148.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0F67_RS03020 (L0F67_03015)	599773..602365	-	2593	Protein_574	DNA mismatch repair protein MutS	-
L0F67_RS03025 (L0F67_03020)	602531..603280	-	750	WP_015674150.1	amino acid ABC transporter ATP-binding protein	-
L0F67_RS03030 (L0F67_03025)	603302..604018	-	717	WP_005605560.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (11-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-86)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14051.07 Da
• Isoelectric Point: 10.2511

>T231185 WP_015674148.1 NZ_CP090556:c599599-599237 [Actinobacillus suis]
VKLVFVELPPFERYRQKHLSDEEYRHFQNELLENPNKGDLIQGTNGLRKIRIADMQRNKGKRGGARVIYYHIVNYSKILL
VTAYGKDEQSDLSNTERNMLANKVKRIVELEIRSSNGKTF

Nucleotide

• Length: 363 bp

>T231185 NZ_CP126113:c2700134-2699784 [Siminovitchia fortis]
TTGGTCGTTAAACGGGGTGATGTGTTTTTCGCCGATTTATCCCCAGTAGTTGGTTCGGAACAAGGCGGAGTCAGGCCGGT
GCTTGTGATCCAGAACGATATTGGAAACCGGTTCAGTCCCACAGTCATTGTTGCAGCCATTACTGCCCAAATCCAAAAAG
CAAAGCTGCCTACACATGTGGAAATTGATTCAAAGCGTTACGGTTTTGAAAGGGATTCAGTCATACTGCTTGAACAGATT
AGAACAATCGACAAGCAGCGTCTTACTGATAAGATTACACATCTGGATGAAGAAATGATGGAGAAAGTGGATGATGCCTT
GCAGATCAGCCTGGGCCTCATCGCTTTTTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11652.60 Da
• Isoelectric Point: 8.5450

>AT231185 WP_015674147.1 NZ_CP090556:c599253-598945 [Actinobacillus suis]
MGKLFDDLMEGLDAYEKHIQGKITLRTTVLEKPEPVELSPSEVKLIREKLNLSQAVFAHKLHTSVRTYQGWEQGKTKPNP
QAVLLLRMVDKSPQLLEQMAQL

Nucleotide

• Length: 309 bp

>AT231185 NZ_CP126113:c2700420-2700139 [Siminovitchia fortis]
GTGTCTGAATCCAGCGCAACAACTGAAATTATCATTCGTTTACCACAGCAGCTCTTGGCAGAGTTGGATGGATTTGTGGA
GCAAGAAAATGTAAATCGAAATGAATTTATCTACCGGGCTACAAAAATGTACATTCGCGAACGAAAAAAACGCCACATAG
TAGAATCAATGAGACGAGGCTACATGGAAATGGCAAAGATCAACCTAGCTATCGCATCGGAGGCTATACAGGCGGAATAT
GAGGCAGAGCATACTGTCGAGCGGCTTGTAAGCGGGGGATGA

Structures

Toxin

• AlphaFold DB: K0G6E5