Detailed information of TA system TA023115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 1747082..1747715
• Replicon: chromosome
• Accession: NC_011312
• Organism: Aliivibrio salmonicida LFI1238

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1E5D3W9
• Locus tag: VSAL_RS08740
• Protein ID: WP_023603298.1
• Coordinates: 1747383..1747715 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: VSAL_RS08735
• Protein ID: WP_012550279.1
• Coordinates: 1747082..1747396 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VSAL_RS08700	1743096..1743371	+	276	WP_044583260.1	DUF1778 domain-containing protein	-
VSAL_RS08705	1743368..1743871	+	504	WP_012550273.1	GNAT family N-acetyltransferase	-
VSAL_RS08710	1744160..1744405	-	246	WP_012550274.1	hypothetical protein	-
VSAL_RS08715	1744395..1744658	-	264	WP_012550275.1	BrnT family toxin	-
VSAL_RS08720	1744838..1745956	-	1119	WP_012550276.1	hypothetical protein	-
VSAL_RS08725	1745949..1746257	-	309	WP_012550277.1	hypothetical protein	-
VSAL_RS08730	1746239..1746901	-	663	WP_012550278.1	hypothetical protein	-
VSAL_RS08735	1747082..1747396	-	315	WP_012550279.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
VSAL_RS08740	1747383..1747715	-	333	WP_023603298.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VSAL_RS08745	1747924..1748373	-	450	WP_044583261.1	hypothetical protein	-
VSAL_RS08750	1748538..1748993	-	456	WP_012550282.1	hypothetical protein	-
VSAL_RS08755	1749138..1749884	-	747	WP_012550283.1	hypothetical protein	-
VSAL_RS08760	1750561..1751307	-	747	WP_012550285.1	hypothetical protein	-
VSAL_RS08765	1751427..1752308	-	882	WP_012548944.1	IS982-like element ISVsa6 family transposase	-
VSAL_RS22800	1752413..1752619	+	207	WP_158306878.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	1744395..1751049	6654

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12934.81 Da
• Isoelectric Point: 10.0960

>T23115 WP_023603298.1 NC_011312:c1747715-1747383 [Aliivibrio salmonicida LFI1238]
MKSIFVESTIFEKYRNEYLSDEEFRLFQAELMSNPKQGDVIQGTGGLRKIRVASKGKGKRGGSRVIYYFLDEKRRFYLLT
IYGKNEMSDLTADQKKQLKAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T23115 NC_011312:c1747715-1747383 [Aliivibrio salmonicida LFI1238]
ATGAAAAGTATATTTGTCGAATCGACCATATTTGAAAAGTACCGTAATGAGTATCTCAGCGATGAAGAGTTTAGACTTTT
TCAAGCTGAATTAATGTCTAATCCGAAACAGGGTGATGTGATTCAAGGTACAGGTGGTTTGCGAAAGATTCGAGTTGCAA
GTAAAGGCAAGGGCAAGCGTGGTGGCTCTCGTGTTATCTATTACTTCCTCGATGAAAAACGTCGTTTCTACTTGCTGACA
ATTTATGGCAAGAATGAAATGTCCGATTTAACCGCAGACCAAAAGAAACAATTAAAGGCTTTTATGGAGGCGTGGCGTAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11763.20 Da
• Isoelectric Point: 6.2155

>AT23115 WP_012550279.1 NC_011312:c1747396-1747082 [Aliivibrio salmonicida LFI1238]
MSNRDLFTELSSAIIEAKEHSEGKLTLKTHQVNDFSELDISPNEIVSIREQFNMSRGVFAHLLHTSSRTLENWEQGRSAP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT23115 NC_011312:c1747396-1747082 [Aliivibrio salmonicida LFI1238]
ATGAGCAATCGTGACCTATTTACAGAGTTAAGTTCAGCTATTATTGAGGCTAAAGAACATTCTGAGGGTAAGTTAACCCT
TAAAACTCATCAAGTTAACGATTTCAGTGAGTTAGATATTTCACCTAATGAAATCGTGAGTATTCGTGAACAATTTAATA
TGTCTCGTGGTGTGTTTGCACATTTACTGCATACATCATCTCGTACATTAGAAAATTGGGAACAAGGGCGTAGCGCACCA
AATGGACAAGCGGTAACCCTTTTAAAATTGGTTCAGCGTCATCCAGAAACCCTGTCACACATCGCTGAGCTATAA

Structures

Toxin

• AlphaFold DB: A0A1E5D3W9