Detailed information of TA system TA231090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4760546..4761300
• Replicon: chromosome
• Accession: NZ_CP090535
• Organism: Salmonella enterica strain 2016089-SE

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: L0F58_RS23185
• Protein ID: WP_000558166.1
• Coordinates: 4760546..4760857 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L0F58_RS23190
• Protein ID: WP_001259011.1
• Coordinates: 4760854..4761300 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0F58_RS23155 (4756204)	4756204..4757106	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
L0F58_RS23160 (4757103)	4757103..4757738	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
L0F58_RS23165 (4757735)	4757735..4758664	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
L0F58_RS23170 (4758711)	4758711..4759001	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
L0F58_RS23175 (4759002)	4759002..4759313	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
L0F58_RS23180 (4759531)	4759531..4760460	+	930	WP_001127703.1	alpha/beta hydrolase	-
L0F58_RS23185 (4760546)	4760546..4760857	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
L0F58_RS23190 (4760854)	4760854..4761300	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
L0F58_RS23195 (4761315)	4761315..4762256	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
L0F58_RS23200 (4762301)	4762301..4762738	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
L0F58_RS23205 (4762735)	4762735..4763607	-	873	WP_000921427.1	virulence factor BrkB family protein	-
L0F58_RS23210 (4763601)	4763601..4764200	-	600	WP_000965695.1	glucose-1-phosphatase	-
L0F58_RS23215 (4764391)	4764391..4765194	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
L0F58_RS23220 (4765228)	4765228..4766124	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T231090 WP_000558166.1 NZ_CP090535:4760546-4760857 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T231090 NZ_CP125961:c760078-759794 [Pseudomonas brassicacearum]
ATGCAGGTTGAGTGGCTGCGTACTGCCCTCAAGAATCTGGACGACGAAGCCGCTTACATTGCGCTGGACAATCCAAAAGC
AGCAGCGCACTTCGTCAAGGCAATCCTCATGAGCGTCGAGCAACTTGCCCAATTCCCGGCGTCCGGTCGTGAAGGCCGCC
TGGCTGGCACCCGTGAATGGGTTGTCCCCGACCGTCCGTACCTGATTCCCTATCGCGTTCGCCACGGGCGCGTGCAAATC
CTGCGACTGTTCCATACGCGACGATTGCCGCCCAACGCCTGGTAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT231090 WP_001259011.1 NZ_CP090535:4760854-4761300 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT231090 NZ_CP125961:c760307-760068 [Pseudomonas brassicacearum]
ATGTCCGTCATGTCATTGCGCTTGCCCGATGAAGTCGCCGAAACCCTGGCGATATTGGCCAAGGCTACCGGGCGCAGCAA
ATCATTCCTGGCCGTCGATGCGCTACGCGAATACCTGGCCCGTGAAGCCTGGCAGATCAAGGAAATCCAAGAAGCCTTGC
ATGAGGCAGATGCAGGTGACTTCGCTAGTCCCGAGGATGTCAACGCGATTGCCGAAAAATGGATGAACAATGCAGGTTGA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90