Detailed information of TA system TA231065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4986468..4987070
• Replicon: chromosome
• Accession: NZ_CP090533
• Organism: Salmonella enterica strain 2017005-SE

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: L0F61_RS24560
• Protein ID: WP_001159630.1
• Coordinates: 4986468..4986779 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L0F61_RS24565
• Protein ID: WP_000362050.1
• Coordinates: 4986780..4987070 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0F61_RS24525 (4981581)	4981581..4982180	+	600	WP_000965695.1	glucose-1-phosphatase	-
L0F61_RS24530 (4982174)	4982174..4983046	+	873	WP_000921427.1	virulence factor BrkB family protein	-
L0F61_RS24535 (4983043)	4983043..4983480	+	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
L0F61_RS24540 (4983525)	4983525..4984466	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
L0F61_RS24545 (4984481)	4984481..4984927	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
L0F61_RS24550 (4984924)	4984924..4985235	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
L0F61_RS24555 (4985321)	4985321..4986250	-	930	WP_001127703.1	alpha/beta hydrolase	-
L0F61_RS24560 (4986468)	4986468..4986779	+	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
L0F61_RS24565 (4986780)	4986780..4987070	+	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
L0F61_RS24570 (4987117)	4987117..4988046	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
L0F61_RS24575 (4988043)	4988043..4988678	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
L0F61_RS24580 (4988675)	4988675..4989577	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T231065 WP_001159630.1 NZ_CP090533:4986468-4986779 [Salmonella enterica]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T231065 NZ_CP125903:c2233126-2232764 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT231065 WP_000362050.1 NZ_CP090533:4986780-4987070 [Salmonella enterica]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT231065 NZ_CP125903:c2233293-2233123 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64