Detailed information of TA system TA231064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4984481..4985235
• Replicon: chromosome
• Accession: NZ_CP090533
• Organism: Salmonella enterica strain 2017005-SE

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: L0F61_RS24550
• Protein ID: WP_000558166.1
• Coordinates: 4984924..4985235 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L0F61_RS24545
• Protein ID: WP_001259011.1
• Coordinates: 4984481..4984927 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0F61_RS24515 (4979657)	4979657..4980553	+	897	WP_001520529.1	sugar kinase	-
L0F61_RS24520 (4980587)	4980587..4981390	+	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
L0F61_RS24525 (4981581)	4981581..4982180	+	600	WP_000965695.1	glucose-1-phosphatase	-
L0F61_RS24530 (4982174)	4982174..4983046	+	873	WP_000921427.1	virulence factor BrkB family protein	-
L0F61_RS24535 (4983043)	4983043..4983480	+	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
L0F61_RS24540 (4983525)	4983525..4984466	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
L0F61_RS24545 (4984481)	4984481..4984927	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
L0F61_RS24550 (4984924)	4984924..4985235	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
L0F61_RS24555 (4985321)	4985321..4986250	-	930	WP_001127703.1	alpha/beta hydrolase	-
L0F61_RS24560 (4986468)	4986468..4986779	+	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
L0F61_RS24565 (4986780)	4986780..4987070	+	291	WP_000362050.1	DNA-binding transcriptional regulator	-
L0F61_RS24570 (4987117)	4987117..4988046	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
L0F61_RS24575 (4988043)	4988043..4988678	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
L0F61_RS24580 (4988675)	4988675..4989577	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T231064 WP_000558166.1 NZ_CP090533:c4985235-4984924 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T231064 NZ_CP125903:c2146049-2145798 [Staphylococcus aureus]
ATGAATAATCGCGAACAAATTGAACAATCAGTTATAAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACGTTTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGCATTAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT231064 WP_001259011.1 NZ_CP090533:c4984927-4984481 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT231064 NZ_CP125903:c2145808-2145626 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGAGCAGGAAAATGACTAA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90