Detailed information of TA system TA023104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4089689..4090286
• Replicon: chromosome
• Accession: NC_011283
• Organism: Klebsiella pneumoniae 342

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: KPK_RS19140
• Protein ID: WP_012542526.1
• Coordinates: 4089969..4090286 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KPK_RS19135
• Protein ID: WP_012542525.1
• Coordinates: 4089689..4089976 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPK_RS19105	4085815..4086063	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
KPK_RS19110	4086080..4086421	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
KPK_RS19115	4086452..4087567	-	1116	WP_162499993.1	MBL fold metallo-hydrolase	-
KPK_RS19120	4087747..4088232	+	486	WP_012542523.1	TetR/AcrR family transcriptional regulator	-
KPK_RS19125	4088232..4088600	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
KPK_RS19130	4088720..4089373	+	654	WP_012542524.1	oxygen-insensitive NAD(P)H nitroreductase	-
KPK_RS19135	4089689..4089976	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
KPK_RS19140	4089969..4090286	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KPK_RS19145	4090471..4091514	-	1044	WP_012542527.1	DUF2157 domain-containing protein	-
KPK_RS19150	4092049..4092915	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
KPK_RS19155	4093024..4094451	+	1428	WP_012542528.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T23104 WP_012542526.1 NC_011283:c4090286-4089969 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T23104 NC_011283:c4090286-4089969 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT23104 WP_012542525.1 NC_011283:c4089976-4089689 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT23104 NC_011283:c4089976-4089689 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2