Detailed information of TA system TA231035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4779956..4780558
• Replicon: chromosome
• Accession: NZ_CP090529
• Organism: Salmonella enterica strain 2008079-SE

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: L0F60_RS23305
• Protein ID: WP_001159630.1
• Coordinates: 4780247..4780558 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L0F60_RS23300
• Protein ID: WP_000362050.1
• Coordinates: 4779956..4780246 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0F60_RS23285 (4777449)	4777449..4778351	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
L0F60_RS23290 (4778348)	4778348..4778983	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
L0F60_RS23295 (4778980)	4778980..4779909	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
L0F60_RS23300 (4779956)	4779956..4780246	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
L0F60_RS23305 (4780247)	4780247..4780558	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
L0F60_RS23310 (4780776)	4780776..4781705	+	930	WP_001127703.1	alpha/beta hydrolase	-
L0F60_RS23315 (4781791)	4781791..4782102	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
L0F60_RS23320 (4782099)	4782099..4782545	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
L0F60_RS23325 (4782560)	4782560..4783501	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
L0F60_RS23330 (4783546)	4783546..4783983	-	438	WP_131166381.1	D-aminoacyl-tRNA deacylase	-
L0F60_RS23335 (4783980)	4783980..4784852	-	873	WP_000921427.1	virulence factor BrkB family protein	-
L0F60_RS23340 (4784846)	4784846..4785445	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T231035 WP_001159630.1 NZ_CP090529:c4780558-4780247 [Salmonella enterica]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T231035 NZ_CP125897:c2141894-2141685 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT231035 WP_000362050.1 NZ_CP090529:c4780246-4779956 [Salmonella enterica]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT231035 NZ_CP125897:c2142199-2141906 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64