Detailed information of TA system TA231011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2351918..2352559
• Replicon: chromosome
• Accession: NZ_CP090526
• Organism: Cupriavidus metallidurans strain ZM16

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q1LE01
• Locus tag: LZQ98_RS10375
• Protein ID: WP_011519196.1
• Coordinates: 2351918..2352202 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LZQ98_RS10380
• Protein ID: WP_011519197.1
• Coordinates: 2352212..2352559 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZQ98_RS10345	2346920..2347321	+	402	WP_224444192.1	RT0821/Lpp0805 family surface protein	-
LZQ98_RS10350	2347417..2348007	-	591	WP_011519191.1	hypothetical protein	-
LZQ98_RS10355	2348130..2348816	-	687	WP_029306340.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
LZQ98_RS10360	2349169..2350059	-	891	WP_224444193.1	helix-turn-helix transcriptional regulator	-
LZQ98_RS10365	2350370..2350588	+	219	WP_008651241.1	YdcH family protein	-
LZQ98_RS10370	2350590..2351741	+	1152	WP_011519195.1	polyphosphate kinase 2	-
LZQ98_RS10375	2351918..2352202	+	285	WP_011519196.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZQ98_RS10380	2352212..2352559	+	348	WP_011519197.1	HigA family addiction module antitoxin	Antitoxin
LZQ98_RS10385	2352674..2353987	+	1314	WP_011519198.1	hemolysin family protein	-
LZQ98_RS10390	2354387..2355724	+	1338	WP_224444194.1	EAL domain-containing protein	-
LZQ98_RS10395	2355884..2355997	+	114	WP_223277463.1	entericidin A/B family lipoprotein	-
LZQ98_RS10400	2356116..2356757	-	642	WP_224444195.1	bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (23-99)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10505.15 Da
• Isoelectric Point: 8.5384

>T231011 WP_011519196.1 NZ_CP090526:2351918-2352202 [Cupriavidus metallidurans]
MIRSLSDKTTAAIYCGQSVHTLPAHIQADALRKLAILDAASSLPDMVAPPANRLEPVLGKRRGHWRVRIKGPWHLCFRFI
NGEAWDVGIAEYEE

Nucleotide

• Length: 285 bp

>T231011 NZ_CP125856:3207841-3209052 [Vibrio parahaemolyticus]
GTGAGTGATAAAGAAAAACGTGTCGTCAGTTTTCAAATTGACGACACTCCCACCGCCGAACAGGCATCAAAAAACTTCCA
AGACGACTATGAGCTGTGCTTCCAAGATTTTCTTGAAAGAACGGTTGATGAAAGTGCCGCTGAAGATTTCTCATACTCCA
ATAACGGAGTTAAGAAAGCAGGGCGACGACTACGCAAGAAAGAAGGCGATCTTAAATCCGCGACTGCGACCATCCAGCAG
TTTCGTGCAGCCCATGAAAAACCACTGAATACTATCGCCTACCTTGTTGGTCGATGTTGTCGAGAGCTTGGGATAGATGT
TAAACCAGTGACACGACTGAAGCGTTTAGAAACCATAGTAGATAAGCTACAGCGTAAATCTCTTGATGGTGAAACTACGA
ATGCGACCTGTGTAACCAATATGAACGATATTGGTGGTTGTCGGGCAATTTTCCCTGATCTTGAATCGTTGGAGAAAGCA
AAAGAGCGTCTGCAAGTTACCGTAGAAAAAGTAGGTCGAATTAAGATTAAGGACATTGATGACTACATCACTAATCCTAA
ACCTAATGACTGCGGATACCGAAGTTTGCATGTTATATATCGTTATGACCATGCCAGCGGTAAAAGTTTTAATATAGAAG
CTCAGCTACGTACTCGTCTTCAGCATCTTTGGGCGACAACTGTAGAAATCGTCGATATTTTGGAAGGTACTAAGATTAAG
ACGCATTCTCATAGCCCAGATGACGACAAAGAGAAGTTGCAGGTTCAGTGGGAAGAGCTACTTTCCATCATGAGTCAGTA
TATTGCTGACGCCGAAGGTGCAATAGAGTTAAGCAATGACAATAGGACTCAGTTTTCAAATAGACTGATTGAATTGAATC
GTGAAATCAATGCATTGAGTCGATTGAGAAGCTTTAAAATCTTATCTGAAAAAGTTGAAGGTTGTCGTGATGGCTCAATA
GAACATGTTCTGTTAGTTATTGACGAAACTTCGTTAGAAATGATTTTTAGTGAAGAGTTTGAGAATTATACCCAAGCTAT
CTCGGTTTACAACGCAGCTGAGAAAATGACTCGTAGTCATGCGAGATTAAACACCCTTCTTATATCTACGAAAAATATGG
GGCAACTATCAGAAGCATACCCCAACTATCTAGGCGACTGTGCTTCATTCATCACGCTGCTTGAAGACGCTATGCAATCG
GGCCAGAATTAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 12691.46 Da
• Isoelectric Point: 7.3198

>AT231011 WP_011519197.1 NZ_CP090526:2352212-2352559 [Cupriavidus metallidurans]
MTINREDLRRADFRSIATGQPIPPAHPGDVLETEFMAPNELSAEALAVALHMAPQRIHDIVRRQRAISPDTALRLARYFG
TSAEFWAGLQTAYALRLARATAGANIERDIQPLTA

Nucleotide

• Length: 348 bp

>AT231011 NZ_CP125856:3207377-3207844 [Vibrio parahaemolyticus]
ATGAAAGCATATCACACATACAGTGCTGTAGACGTTGCATGGAGCTTGCTTAAGCATGCGAAAGAGCAAGGTAAATGCTT
CTCCAATTTGCAGCTACAAAAACTTACCTATGTTTGCCACGGTTTATCATTGGCACACTTCCAGCGTCCTCTTGTTGTGG
ATGACGTATTTGCTTGGAAATTTGGTCCCGTAGTTCCTTCTGTCTATTTTCGCTTTAAATCGTATGGCAGTAACGTGATC
ACCGAACAATGTGATGTGGTGTTAGATCATGAAAGTGAATCTATTGTCAAAGATGTAGTATCACAACTGGGGCATTTAAC
CGGCCCGCAGCTTGTAGATCTGACTCACCGTGAGGGTAGTCCATGGCATCAAGTATGGGATGGCACACATCAAAAAGTGA
TTCCTGACCATTTGATTCAAGCTCATTACACTCAGATCAAACAATCAGGGCGAACAGCTAGCCTGTGA

Structures

Toxin

• AlphaFold DB: A0A132H834