Detailed information of TA system TA230974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 460334..460901
• Replicon: chromosome
• Accession: NZ_CP090513
• Organism: Xylella fastidiosa subsp. multiplex strain Red Oak 8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LZ751_RS01880
• Protein ID: WP_004085174.1
• Coordinates: 460334..460615 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: LZ751_RS01885
• Protein ID: WP_012337645.1
• Coordinates: 460626..460901 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ751_RS01850 (LZ751_01850)	456196..457788	-	1593	WP_012337641.1	tail fiber domain-containing protein	-
LZ751_RS01855 (LZ751_01855)	457796..458353	-	558	WP_012337642.1	phage tail protein I	-
LZ751_RS01860 (LZ751_01860)	458346..459239	-	894	WP_012337643.1	baseplate J/gp47 family protein	-
LZ751_RS01865 (LZ751_01865)	459239..459643	-	405	WP_012337644.1	GPW/gp25 family protein	-
LZ751_RS01870 (LZ751_01870)	459685..459966	+	282	WP_004087027.1	XRE family transcriptional regulator	-
LZ751_RS01875 (LZ751_01875)	459978..460277	+	300	WP_004087025.1	HigA family addiction module antitoxin	-
LZ751_RS01880 (LZ751_01880)	460334..460615	+	282	WP_004085174.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZ751_RS01885 (LZ751_01885)	460626..460901	+	276	WP_012337645.1	HigA family addiction module antitoxin	Antitoxin
LZ751_RS01890 (LZ751_01890)	460906..461493	-	588	WP_012337646.1	phage baseplate assembly protein V	-
LZ751_RS01895 (LZ751_01895)	461490..462032	-	543	WP_012337647.1	hypothetical protein	-
LZ751_RS01900 (LZ751_01900)	462008..462529	-	522	WP_012337648.1	hypothetical protein	-
LZ751_RS01905 (LZ751_01905)	462526..462849	-	324	WP_012337649.1	hypothetical protein	-
LZ751_RS01910 (LZ751_01910)	462849..463109	-	261	WP_012337650.1	hypothetical protein	-
LZ751_RS01915 (LZ751_01915)	463127..463927	-	801	WP_234556088.1	phage major capsid protein	-
LZ751_RS01920 (LZ751_01920)	463996..465000	-	1005	WP_234556080.1	HK97 family phage prohead protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	446882..481254	34372

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10610.17 Da
• Isoelectric Point: 9.8829

>T230974 WP_004085174.1 NZ_CP090513:460334-460615 [Xylella fastidiosa subsp. multiplex]
MIKSFRHKGIQQFFLVGSTAGIQTKHAAKLRIQLTALESAKRPEDMNAPGWKLHPLKGADLKGHWSIWVNGNYRLTFAFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T230974 NZ_CP125762:c1572117-1571938 [Bacillus subtilis]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATTCATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9982.56 Da
• Isoelectric Point: 10.2184

>AT230974 WP_012337645.1 NZ_CP090513:460626-460901 [Xylella fastidiosa subsp. multiplex]
MKRMHNPAHPGEVLREYLGDLTVTNAAAKLGVSRVALSRILNGTNGISADMALRLEDALGTSAEMWAAMQAKYDLWVASQ
QPRPKITRLHV

Nucleotide

• Length: 276 bp

>AT230974 NZ_CP125762:1571792-1572014 [Bacillus subtilis]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAACTACCCTTTAATAG
GAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC