Detailed information of TA system TA230969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 2491911..2492617
• Replicon: chromosome
• Accession: NZ_CP090511
• Organism: Xylella fastidiosa subsp. multiplex strain Oak 35874

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: LZ754_RS11545
• Protein ID: WP_128283742.1
• Coordinates: 2492315..2492617 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: -
• Locus tag: LZ754_RS11540
• Protein ID: WP_060872143.1
• Coordinates: 2491911..2492312 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ754_RS11510 (LZ754_11510)	2487748..2489621	+	1874	Protein_2262	phage major capsid protein	-
LZ754_RS11515 (LZ754_11515)	2489639..2489899	+	261	WP_012382619.1	hypothetical protein	-
LZ754_RS11520 (LZ754_11520)	2489899..2490222	+	324	WP_038210491.1	hypothetical protein	-
LZ754_RS11525 (LZ754_11525)	2490219..2490740	+	522	WP_234499820.1	hypothetical protein	-
LZ754_RS11530 (LZ754_11530)	2490716..2491258	+	543	WP_038210495.1	hypothetical protein	-
LZ754_RS11535 (LZ754_11535)	2491255..2491842	+	588	WP_234499821.1	phage baseplate assembly protein V	-
LZ754_RS11540 (LZ754_11540)	2491911..2492312	-	402	WP_060872143.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
LZ754_RS11545 (LZ754_11545)	2492315..2492617	-	303	WP_128283742.1	type II toxin-antitoxin system MqsR family toxin	Toxin
LZ754_RS11550 (LZ754_11550)	2492861..2493184	+	324	WP_228304176.1	GPW/gp25 family protein	-
LZ754_RS11555 (LZ754_11555)	2493184..2494077	+	894	WP_040123073.1	baseplate J/gp47 family protein	-
LZ754_RS11560 (LZ754_11560)	2494070..2494627	+	558	WP_234499822.1	phage tail protein I	-
LZ754_RS11565 (LZ754_11565)	2494635..2496227	+	1593	WP_234499823.1	hypothetical protein	-
LZ754_RS11570 (LZ754_11570)	2496292..2497470	+	1179	WP_234499824.1	phage tail sheath family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2452209..2502823	50614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (74-127)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10958.74 Da
• Isoelectric Point: 8.0251

>T230969 WP_128283742.1 NZ_CP090511:c2492617-2492315 [Xylella fastidiosa subsp. multiplex]
MEKGTPHYKLPVVKALVKAGKVRATATAYQGARELGIKDLTGMCAVVLALTSADFYKSMTTYTDHTVFQDVYHAKTASGD
EVYLKLTVIDDVLIVSFKEL

Nucleotide

• Length: 303 bp

>T230969 NZ_CP125734:c5199754-5199371 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 15004.12 Da
• Isoelectric Point: 7.1650

>AT230969 WP_060872143.1 NZ_CP090511:c2492312-2491911 [Xylella fastidiosa subsp. multiplex]
MRCPCCGAAELIHDTRDMLYTYKSETTSIPTVTGDFCPACGEVVLDREHGDRYSELVGLFQRQVNSAYVDPGYITKVRRK
LDLDQRQAAELFGGGVNAFSRYENGKTKPPLSLVKLFKLLDRHPDLLNEVKSF

Nucleotide

• Length: 402 bp

>AT230969 NZ_CP125734:c5199996-5199754 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA