Detailed information of TA system TA023091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 353684..354330
• Replicon: chromosome
• Accession: NC_011283
• Organism: Klebsiella pneumoniae 342

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KPK_RS01580
• Protein ID: WP_012540399.1
• Coordinates: 353684..354031 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1F2LZT5
• Locus tag: KPK_RS01585
• Protein ID: WP_008806992.1
• Coordinates: 354031..354330 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPK_RS01570	349571..351004	+	1434	WP_004202133.1	glycogen synthase GlgA	-
KPK_RS01575	351022..353469	+	2448	WP_008806990.1	glycogen phosphorylase	-
KPK_RS01580	353684..354031	+	348	WP_012540399.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KPK_RS01585	354031..354330	+	300	WP_008806992.1	helix-turn-helix transcriptional regulator	Antitoxin
KPK_RS01590	354393..355901	-	1509	WP_008806993.1	glycerol-3-phosphate dehydrogenase	-
KPK_RS01595	356106..356435	+	330	WP_004202138.1	thiosulfate sulfurtransferase GlpE	-
KPK_RS01600	356486..357316	+	831	WP_008806994.1	rhomboid family intramembrane serine protease GlpG	-
KPK_RS01605	357366..358124	+	759	WP_008806995.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13505.50 Da
• Isoelectric Point: 5.6749

>T23091 WP_012540399.1 NC_011283:353684-354031 [Klebsiella variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFGPQLGRPYVDTVKDSTFQDMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T23091 NC_011283:353684-354031 [Klebsiella variicola]
ATGTGGGACGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGGTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGG
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10814.61 Da
• Isoelectric Point: 10.1464

>AT23091 WP_008806992.1 NC_011283:354031-354330 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT23091 NC_011283:354031-354330 [Klebsiella variicola]
ATGGCCACGCTAAAAGAACTTATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGATCCGCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTTACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1F2LZT5