Detailed information of TA system TA230901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 332221..332731
• Replicon: chromosome
• Accession: NZ_CP090486
• Organism: Photobacterium damselae subsp. damselae strain AS-16-0963-1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A2T3QIJ8
• Locus tag: IHC93_RS16545
• Protein ID: WP_036765839.1
• Coordinates: 332471..332731 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A2T3QIT0
• Locus tag: IHC93_RS16540
• Protein ID: WP_036765838.1
• Coordinates: 332221..332481 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHC93_RS16515 (IHC93_16515)	327758..328513	+	756	WP_068968727.1	TPM domain-containing protein	-
IHC93_RS16520 (IHC93_16520)	328516..329133	+	618	WP_237041939.1	TPM domain-containing protein	-
IHC93_RS16525 (IHC93_16525)	329303..329530	+	228	WP_036765835.1	YgjV family protein	-
IHC93_RS16530 (IHC93_16530)	329537..330271	+	735	WP_106223052.1	hypothetical protein	-
IHC93_RS16535 (IHC93_16535)	330346..332028	+	1683	WP_237042058.1	RluA family pseudouridine synthase	-
IHC93_RS16540 (IHC93_16540)	332221..332481	+	261	WP_036765838.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IHC93_RS16545 (IHC93_16545)	332471..332731	+	261	WP_036765839.1	Txe/YoeB family addiction module toxin	Toxin
IHC93_RS16550 (IHC93_16550)	332789..333043	-	255	WP_036765840.1	hypothetical protein	-
IHC93_RS16555 (IHC93_16555)	333055..333939	-	885	WP_005305889.1	AraC family transcriptional regulator	-
IHC93_RS16560 (IHC93_16560)	334099..335247	+	1149	WP_086368441.1	iron-containing alcohol dehydrogenase	-
IHC93_RS16565 (IHC93_16565)	335432..336268	+	837	WP_005305896.1	HNH endonuclease signature motif containing protein	-
IHC93_RS16570 (IHC93_16570)	336330..337232	-	903	WP_237041941.1	LysR substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10319.86 Da
• Isoelectric Point: 9.7118

>T230901 WP_036765839.1 NZ_CP090486:332471-332731 [Photobacterium damselae subsp. damselae]
MMINFTPNAWDDYLYWQSQDKKTLKRINKLIENCVRTPFEGIGKPEPLKDNLSGYWSRRIDDTHRLVYKATQDTLVIVAV
RFHYQK

Nucleotide

• Length: 261 bp

>T230901 NZ_CP125654:490252-490602 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9718.89 Da
• Isoelectric Point: 4.3167

>AT230901 WP_036765838.1 NZ_CP090486:332221-332481 [Photobacterium damselae subsp. damselae]
MTVLSFSEVRGNFKKVCDQVVDDCDAVVIHRRDADNVVLMSESEFNSWKETMYLMSNPVNAANLLESIKQAECGNVQTHE
LLSHDD

Nucleotide

• Length: 261 bp

>AT230901 NZ_CP125654:489965-490246 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A2T3QIJ8

Antitoxin

• AlphaFold DB: A0A2T3QIT0