Detailed information of TA system TA230900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2618499..2619151
• Replicon: chromosome
• Accession: NZ_CP090485
• Organism: Photobacterium damselae subsp. damselae strain AS-16-0963-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2T3QK61
• Locus tag: IHC93_RS12335
• Protein ID: WP_036764377.1
• Coordinates: 2618499..2618846 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IHC93_RS12340
• Protein ID: WP_036764379.1
• Coordinates: 2618849..2619151 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHC93_RS12325 (IHC93_12325)	2614573..2615961	-	1389	WP_005301055.1	DNA repair protein RadA	-
IHC93_RS12330 (IHC93_12330)	2616056..2618410	+	2355	WP_237041117.1	PilZ domain-containing protein	-
IHC93_RS12335 (IHC93_12335)	2618499..2618846	+	348	WP_036764377.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IHC93_RS12340 (IHC93_12340)	2618849..2619151	+	303	WP_036764379.1	helix-turn-helix transcriptional regulator	Antitoxin
IHC93_RS12345 (IHC93_12345)	2619239..2620446	+	1208	Protein_2293	IS256 family transposase	-
IHC93_RS12350 (IHC93_12350)	2620626..2621627	-	1002	WP_005301064.1	phosphoserine phosphatase	-
IHC93_RS12355 (IHC93_12355)	2621817..2622431	+	615	WP_005301066.1	AhpA/YtjB family protein	-
IHC93_RS12360 (IHC93_12360)	2622557..2623282	-	726	WP_005301070.1	purine-nucleoside phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13097.99 Da
• Isoelectric Point: 6.4672

>T230900 WP_036764377.1 NZ_CP090485:2618499-2618846 [Photobacterium damselae subsp. damselae]
MWTVITTDLFDQWFDSQSDDTQEKVLAGLVALQRGGPTIGRPLVDTVNASKYGNMKELRVQHRGEPIRAFFAFDPLRQAI
VLCAGNKGGNEKRFYKQMIPLADLEFTKHLEELEK

Nucleotide

• Length: 348 bp

>T230900 NZ_CP125653:415246-415518 [Rhizobium sp. BT04]
ATGAAGTTGCTCTGGACCCCGAATTCTTGGGAAGAATACGAATACTGGCAGAAAACGGACCAGAAGATGGTCGAGAAAAT
CAACGAGCTGCTCAGGGATACAAAAAGGTCGCCGTTCAAAGGTCTCGGCAAGCCAGAGCCGCTCAAGGGCGATCTCTCCG
GTTTTTGGTCACGGCGCATTCTGGGAGAGCATCGGCTCGTGTATTGCGTCACAGGCAAGGGTTCCAACCAGCAGTTGGAA
ATCATCCAGTGCCGATTTCATTATGAAAAATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11220.85 Da
• Isoelectric Point: 4.6657

>AT230900 WP_036764379.1 NZ_CP090485:2618849-2619151 [Photobacterium damselae subsp. damselae]
MARNLNDMLASRSQESQDRIAEMADEMLLEVQFQAIREELEITQAELARNMGISQPSVVAIEQRGNDVKLSTIKRYIEAM
GGKMSINIELPTGKHVGFNI

Nucleotide

• Length: 303 bp

>AT230900 NZ_CP125653:414993-415244 [Rhizobium sp. BT04]
ATGGCACATGTTCGTTTGACCGAATTCCGGCAGAACATCGCAACCTATTTCGATCAGGTCCTGTCTTCGCGGGCTCCTCT
GCTCGTGACCCGTCAAGGTTCGGAAGCGATGGTCTTGATCGCCGAAAGCGAGTACGAGAGCATGCAAGAGACATTGCACC
TGCTCTCGACGCCTGCCAATTCTGCCCGGTTACGCGAGAGCATCGGGCAGCTTCGTAATGGGCAGACGATAGAGAACGAT
CCAACTCTCTGA

Structures

Toxin

• AlphaFold DB: A0A2T3QK61