Detailed information of TA system TA230877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 15210..15884
• Replicon: plasmid p3
• Accession: NZ_CP090471
• Organism: Klebsiella pneumoniae strain kp7450

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LMS5
• Locus tag: LZ687_RS27310
• Protein ID: WP_032720638.1
• Coordinates: 15210..15533 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LZ687_RS27315
• Protein ID: WP_032720637.1
• Coordinates: 15582..15884 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ687_RS27280 (LZ687_27280)	10430..10753	+	324	WP_019725650.1	hypothetical protein	-
LZ687_RS27285 (LZ687_27285)	11173..11805	+	633	WP_001567369.1	hypothetical protein	-
LZ687_RS27290 (LZ687_27290)	11834..13237	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
LZ687_RS27295 (LZ687_27295)	13551..14153	+	603	Protein_17	transposase	-
LZ687_RS27300 (LZ687_27300)	14222..14649	-	428	Protein_18	DNA-binding protein	-
LZ687_RS27305 (LZ687_27305)	14627..15032	+	406	Protein_19	DUF4113 domain-containing protein	-
LZ687_RS27310 (LZ687_27310)	15210..15533	+	324	WP_032720638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZ687_RS27315 (LZ687_27315)	15582..15884	+	303	WP_032720637.1	NadS family protein	Antitoxin
LZ687_RS27320 (LZ687_27320)	15927..16094	-	168	WP_153591440.1	hypothetical protein	-
LZ687_RS27325 (LZ687_27325)	16282..16488	-	207	WP_153591439.1	hypothetical protein	-
LZ687_RS27330 (LZ687_27330)	16485..16868	-	384	WP_032720636.1	hypothetical protein	-
LZ687_RS27335 (LZ687_27335)	16973..17545	-	573	WP_032720635.1	GNAT family N-acetyltransferase	-
LZ687_RS27340 (LZ687_27340)	17547..18359	-	813	WP_223203129.1	TSUP family transporter	-
LZ687_RS27345 (LZ687_27345)	18371..19291	-	921	WP_050548604.1	TauD/TfdA family dioxygenase	-
LZ687_RS27350 (LZ687_27350)	19594..20088	-	495	WP_044266755.1	hypothetical protein	-
LZ687_RS27355 (LZ687_27355)	20119..20691	-	573	WP_044266753.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / qnrB1 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15	-	1..135574	135574

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12372.29 Da
• Isoelectric Point: 9.6240

>T230877 WP_032720638.1 NZ_CP090471:15210-15533 [Klebsiella pneumoniae]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDELSEKEKAVMKALTQQLK

Nucleotide

• Length: 324 bp

>T230877 NZ_CP125620:3160704-3160967 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11211.12 Da
• Isoelectric Point: 7.3611

>AT230877 WP_032720637.1 NZ_CP090471:15582-15884 [Klebsiella pneumoniae]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRVPSGIALK
MLCLIEREPALINTLRGIHV

Nucleotide

• Length: 303 bp

>AT230877 NZ_CP125620:3160419-3160700 [Mycobacterium tuberculosis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LMS5