Detailed information of TA system TA230852

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 119863..120116
• Replicon: plasmid p2
• Accession: NZ_CP090464
• Organism: Klebsiella pneumoniae strain kp5152

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LZ688_RS28745
• Protein ID: WP_001312851.1
• Coordinates: 119863..120012 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	120057..120116 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ688_RS28705 (115222)	115222..115637	-	416	Protein_138	IS1-like element IS1B family transposase	-
LZ688_RS28710 (115886)	115886..116287	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
LZ688_RS28715 (116220)	116220..116477	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
LZ688_RS28720 (116570)	116570..117223	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
LZ688_RS28725 (118162)	118162..119019	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
LZ688_RS28735 (119038)	119038..119216	-	179	Protein_143	protein CopA/IncA	-
LZ688_RS28740 (119331)	119331..119579	-	249	WP_000083837.1	replication regulatory protein RepA	-
LZ688_RS28745 (119863)	119863..120012	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (120057)	120057..120116	+	60	NuclAT_1	-	Antitoxin
- (120057)	120057..120116	+	60	NuclAT_1	-	Antitoxin
- (120057)	120057..120116	+	60	NuclAT_1	-	Antitoxin
- (120057)	120057..120116	+	60	NuclAT_1	-	Antitoxin
LZ688_RS28750 (120317)	120317..120649	-	333	WP_152916585.1	hypothetical protein	-
LZ688_RS28755 (120711)	120711..121310	-	600	WP_032083981.1	PIN domain-containing protein	-
LZ688_RS28760 (121696)	121696..121896	-	201	WP_015059022.1	hypothetical protein	-
LZ688_RS28765 (122028)	122028..122588	-	561	WP_000139328.1	fertility inhibition protein FinO	-
LZ688_RS28770 (122643)	122643..123389	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LZ688_RS28775 (123409)	123409..123609	-	201	WP_072354025.1	hypothetical protein	-
LZ688_RS28780 (123634)	123634..124338	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LZ688_RS28785 (124393)	124393..124758	-	366	Protein_153	type IV conjugative transfer system protein TraE	-
LZ688_RS28790 (124778)	124778..125083	-	306	Protein_154	type IV conjugative transfer system protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / blaCTX-M-65	-	1..134477	134477
	-	flank	IS/Tn	-	-	123634..124338	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T230852 WP_001312851.1 NZ_CP090464:c120012-119863 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T230852 NZ_CP125620:1765304-1765708 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT230852 NZ_CP090464:120057-120116 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin