Detailed information of TA system TA230849

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83702..84128
• Replicon: plasmid p2
• Accession: NZ_CP090464
• Organism: Klebsiella pneumoniae strain kp5152

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LZ688_RS28490
• Protein ID: WP_001372321.1
• Coordinates: 83702..83827 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	83904..84128 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ688_RS28460 (78970)	78970..79674	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LZ688_RS28465 (79955)	79955..80338	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LZ688_RS28470 (80615)	80615..81262	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
LZ688_RS28475 (81559)	81559..82380	-	822	WP_001234445.1	DUF932 domain-containing protein	-
LZ688_RS28480 (82491)	82491..82787	-	297	WP_001272251.1	hypothetical protein	-
LZ688_RS28485 (83087)	83087..83383	+	297	Protein_94	hypothetical protein	-
LZ688_RS28490 (83702)	83702..83827	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LZ688_RS28495 (83769)	83769..83918	-	150	Protein_96	plasmid maintenance protein Mok	-
- (83904)	83904..84128	-	225	NuclAT_0	-	Antitoxin
- (83904)	83904..84128	-	225	NuclAT_0	-	Antitoxin
- (83904)	83904..84128	-	225	NuclAT_0	-	Antitoxin
- (83904)	83904..84128	-	225	NuclAT_0	-	Antitoxin
LZ688_RS28500 (84140)	84140..84859	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
LZ688_RS28505 (84856)	84856..85290	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LZ688_RS28510 (85359)	85359..87382	-	2024	Protein_99	ParB/RepB/Spo0J family partition protein	-
LZ688_RS28515 (87443)	87443..87676	-	234	WP_000006003.1	DUF905 family protein	-
LZ688_RS28520 (87734)	87734..88261	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
LZ688_RS28525 (88563)	88563..89018	+	456	Protein_102	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / blaCTX-M-65	-	1..134477	134477

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T230849 WP_001372321.1 NZ_CP090464:c83827-83702 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T230849 NZ_CP125620:1539833-1540654 [Mycobacterium tuberculosis]
GTGGTGGTAGCCCTCGTAGGCTCAGCAATCGTGGACTTGCATTCACGACCACCGTGGTCGAACAACGCGGTGCGTCGTCT
TGGCGTGGCACTGCGCGACGGAGTTGACCCGCCGGTCGACTGCCCGTCGTACGCCGAGGTGATGCTGTGGCATGCGGACT
TGGCCGCCGAAGTCCAGGACCGGATCGAGGGCCGGAGTTGGTCTGCGTCGGAGTTATTGGTTACCTCACGTGCGAAGAGC
CAAGACACCCTGCTAGCAAAGCTGCGGCGTCGGCCTTACCTGCAACTGAACACCATCCAAGACATCGCAGGTGTCCGCAT
CGATGCCGACCTCCTGCTGGGCGAGCAGACGAGACTTGCTCGCGAGATCGCCGACCACTTCGGTGCTGACCAGCCCGCTA
TTCATGATCTGCGTGACCACCCGCACGCCGGCTACCGGGCCGTTCATGTCTGGCTTCGGTTACCTGCCGGTCGTGTCGAG
ATACAGATTCGCACCATTTTGCAGAGCCTGTGGGCCAACTTCTACGAGCTTCTCGCTGACGCGTACGGTCGGGGCATCCG
CTATGACGAGCGGCCGGAGCAGCTAGCGGCCGGCGTTGTCCCGGCACAGCTTCAAGAGCTGGTAGGGGTTATGCAAGACG
CTTCAGCGGATCTGGCGATGCATGAAGCCGAGTGGCAACACTGTGCAGAGATCGAATACCCCGGCCAGCGGGCGATGGCG
CTTGGCGAGGCGAGCAAGAACAAGGCGACGGTGCTCGCAACGACCAAGTTTAGGCTGGAAAGGGCCATCAATGAGGCCGA
GTCGGCAGGGGGAGGTGGGTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT230849 NZ_CP090464:c84128-83904 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin